Magnetic bead technology for viral RNA extraction from serum in blood bank screening

被引：0

作者：

Ambrozio Albertoni, Guilherme ^{[1
]}

Arnoni, Carine Prisco ^{[1
]}

Barboza Araujo, Patricia Regina ^{[1
]}

Andrade, Sheila Siqueira ^{[1
]}

Carvalho, Fabricio Oliveira ^{[1
]}

Batista Castello Girao, Manoel Joao ^{[1
]}

Schor, Nestor ^{[2
]}

Barreto, Jose Augusto ^{[1
]}

机构：

[1] Colsan Assoc Beneficente Coleta Sangue, Sao Paulo, Brazil

[2] Univ Fed Sao Paulo UNIFESP, Sao Paulo, SP, Brazil

来源：

BRAZILIAN JOURNAL OF INFECTIOUS DISEASES | 2011年 / 15卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

HCV; HIV; infection; reverse transcriptase polymerase chain reaction; blood banks; HUMAN-IMMUNODEFICIENCY-VIRUS; HEPATITIS-C VIRUS; NUCLEIC-ACIDS; SAO-PAULO; PURIFICATION; TRANSFUSION; HCV; DNA; INFECTIONS; DONATIONS;

D O I：

暂无

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of Sao Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. Objective: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase polymerase chain reaction (RT-PCR) one-step. Methods: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. Results: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. Conclusion: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products.

引用

页码：547 / 552

页数：6

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