Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp.

被引:18
|
作者
Techapun, C
Sinsuwongwat, S
Poosaran, N
Watanabe, M
Sasaki, K [1 ]
机构
[1] Hiroshima Kokusai Gakuin Univ, Hiroshima 7390321, Japan
[2] Chiang Mai Univ, Fac Agroind, Dept Biotechnol, Chiangmai 50100, Thailand
关键词
agricultural wastes; Streptomyces sp; thermotolerance; xylanase;
D O I
10.1023/A:1012448229555
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
1444 microorganisms were isolated from soil samples from the northern Thai and screened at 55 degreesC by using basal medium supplemented with 1% carboxymethyl cellulose as a sole carbon source. One isolate, Streptomyces Ab106, had a high activity of a cellulase-free xylanase also without mannanase activity. The maximum cellulase-free xylanase activities of 3.5, 3.3, 3.1 and 2.7 IU were after growth of the organism with 1% (w/v) corn hull, corncob, bagasse and oat spelt xylan, respectively, at 55 degreesC for 6 days, respectively. The activity was more than 5 times higher than that at 35 degreesC.
引用
收藏
页码:1685 / 1689
页数:5
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