The Use of Peptide Markers of Carp and Herring Allergens as an Example of Detection of Sequenced and Non-Sequenced Proteins

被引:8
作者
Bucholska, Justyna [1 ]
Minkiewicz, Piotr [1 ]
机构
[1] Univ Warmia & Mazury, Fac Food Sci, Dept Food Biochem, Plac Cieszynski 1, PL-10726 Olsztyn, Poland
关键词
allergens; proteins; fish; markers; peptides; mass spectrometry; MASS-SPECTROMETRY METHODS; HIGH-RESOLUTION; LIQUID-CHROMATOGRAPHY; PROTEOMIC ANALYSIS; MILK-PROTEINS; FOOD ALLERGEN; COMMON; IDENTIFICATION; PREDICTION; QUANTITATION;
D O I
10.17113/ftb.54.03.16.4244
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The objective of this study is to identify fish protein markers for detecting multiple species based on a comparative proteomic approach that relies on fragments with identical sequences. The possibilities and challenges of the use of peptides obtained from carp (Cyprinus carpio) and herring (Clupea harengus) proteins are discussed. A bioinformatic analysis was followed by an LC-MS/MS experiment to identify markers predicting the presence of fish allergenic proteins. Selected myosin peptides were found in carp protein hydrolysates with known sequences and in herring protein hydrolysates with unknown sequences. The results obtained for carp and herring proteins myosin and parvalbumin indicate that proteins with unknown sequences can be identified by peptide markers. Such markers can be designed by disregarding the principle that peptides should be unique (present in one sequence). The challenge is to determine a group of proteins that can be detected by peptide identification.
引用
收藏
页码:266 / 274
页数:9
相关论文
共 46 条
  • [1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [2] EVALLER:: a web server for in silico assessment of potential protein allergenicity
    Barrio, Alvaro Martinez
    Soeria-Atmadja, Daniel
    Nister, Anders
    Gustafsson, Mats G.
    Hammerling, Ulf
    Bongcam-Rudloff, Erik
    [J]. NUCLEIC ACIDS RESEARCH, 2007, 35 : W694 - W700
  • [3] UniProt: a hub for protein information
    Bateman, Alex
    Martin, Maria Jesus
    O'Donovan, Claire
    Magrane, Michele
    Apweiler, Rolf
    Alpi, Emanuele
    Antunes, Ricardo
    Arganiska, Joanna
    Bely, Benoit
    Bingley, Mark
    Bonilla, Carlos
    Britto, Ramona
    Bursteinas, Borisas
    Chavali, Gayatri
    Cibrian-Uhalte, Elena
    Da Silva, Alan
    De Giorgi, Maurizio
    Dogan, Tunca
    Fazzini, Francesco
    Gane, Paul
    Cas-tro, Leyla Garcia
    Garmiri, Penelope
    Hatton-Ellis, Emma
    Hieta, Reija
    Huntley, Rachael
    Legge, Duncan
    Liu, Wudong
    Luo, Jie
    MacDougall, Alistair
    Mutowo, Prudence
    Nightin-gale, Andrew
    Orchard, Sandra
    Pichler, Klemens
    Poggioli, Diego
    Pundir, Sangya
    Pureza, Luis
    Qi, Guoying
    Rosanoff, Steven
    Saidi, Rabie
    Sawford, Tony
    Shypitsyna, Aleksandra
    Turner, Edward
    Volynkin, Vladimir
    Wardell, Tony
    Watkins, Xavier
    Zellner, Hermann
    Cowley, Andrew
    Figueira, Luis
    Li, Weizhong
    McWilliam, Hamish
    [J]. NUCLEIC ACIDS RESEARCH, 2015, 43 (D1) : D204 - D212
  • [4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [5] Purification, biochemical, and immunological characterisation of a major food allergen: different immunoglobulin E recognition of the apo- and calcium-bound forms of carp parvalbumin
    Bugajska-Schretter, A
    Grote, M
    Vangelista, L
    Valent, P
    Sperr, WR
    Rumpold, H
    Pastore, A
    Reichelt, R
    Valenta, R
    Spitzauer, S
    [J]. GUT, 2000, 46 (05) : 661 - 669
  • [6] Use of Proteomics and Peptidomics Methods in Food Bioactive Peptide Science and Engineering
    Carrasco-Castilla, Janet
    Javier Hernandez-Alvarez, Alan
    Jimenez-Martinez, Cristian
    Fidel Gutierrez-Lopez, Gustavo
    Davila-Ortiz, Gloria
    [J]. FOOD ENGINEERING REVIEWS, 2012, 4 (04) : 224 - 243
  • [7] Identification of commercial hake and grenadier species by proteomic analysis of the parvalbumin fraction
    Carrera, Monica
    Canas, Benito
    Pineiro, Carmen
    Vazquez, Jesus
    Gallardo, Jose Manuel
    [J]. PROTEOMICS, 2006, 6 (19) : 5278 - 5287
  • [8] Proteomics for the assessment of quality and safety of fishery products
    Carrera, Monica
    Canas, Benito
    Gallardo, Jose M.
    [J]. FOOD RESEARCH INTERNATIONAL, 2013, 54 (01) : 972 - 979
  • [9] Mass spectrometry in food proteomics: a tutorial
    Cunsolo, Vincenzo
    Muccilli, Vera
    Saletti, Rosaria
    Foti, Salvatore
    [J]. JOURNAL OF MASS SPECTROMETRY, 2014, 49 (09): : 768 - 784
  • [10] Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity and ACE Inhibitory Peptides of Salmon (Salmo salar) Protein Hydrolysates Obtained by Human and Porcine Gastrointestinal Enzymes
    Darewicz, Malgorzata
    Borawska, Justyna
    Vegarud, Gerd E.
    Minkiewicz, Piotr
    Iwaniak, Anna
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2014, 15 (08): : 14077 - 14101