The bacA gene, which determines bacitracin susceptibility in Streptococcus pneumoniae and Staphylococcus aureus, is also required for virulence

被引：70

作者：

Chalker, AF ^{[1
]}

Ingraham, KA ^{[1
]}

Lunsford, RD ^{[1
]}

Bryant, AP ^{[1
]}

Bryant, J ^{[1
]}

Wallis, NG ^{[1
]}

Broskey, JP ^{[1
]}

Pearson, SC ^{[1
]}

Holmes, DJ ^{[1
]}

机构：

[1] SmithKline Beecham Pharmaceut, Antiinfect Res UP1345, Collegeville, PA 19426 USA

来源：

MICROBIOLOGY-SGM | 2000年 / 146卷

关键词：

bacitracin; bacA; undecaprenol kinase; Staphylococcus aureus; Streptococcus pneumoniae;

D O I：

10.1099/00221287-146-7-1547

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Homologues of Escherichia coli bacA. encoding extremely hydrophobic proteins, were identified in the genomes of Staphylococcus aureus and Streptococcus pneumoniae. Allelic replacement mutagenesis demonstrated that the gene is not essential for in vitro growth in either organism, and the mutants showed no significant changes in growth rate or morphology. The Staph. aureus bacA mutant showed slightly reduced virulence in a mouse model of infection and an eightfold increase in bacitracin susceptibility. However, a Strep. pneumoniae bacA mutant was highly attenuated in a mouse model of infection, and demonstrated an increase in susceptibility to bacitracin of up to 160 000-fold. These observations are consistent with the previously proposed role of BacA protein as undecaprenol kinase.

引用

页码：1547 / 1553

页数：7

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