The bacA gene, which determines bacitracin susceptibility in Streptococcus pneumoniae and Staphylococcus aureus, is also required for virulence

被引:69
作者
Chalker, AF [1 ]
Ingraham, KA [1 ]
Lunsford, RD [1 ]
Bryant, AP [1 ]
Bryant, J [1 ]
Wallis, NG [1 ]
Broskey, JP [1 ]
Pearson, SC [1 ]
Holmes, DJ [1 ]
机构
[1] SmithKline Beecham Pharmaceut, Antiinfect Res UP1345, Collegeville, PA 19426 USA
来源
MICROBIOLOGY-SGM | 2000年 / 146卷
关键词
bacitracin; bacA; undecaprenol kinase; Staphylococcus aureus; Streptococcus pneumoniae;
D O I
10.1099/00221287-146-7-1547
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Homologues of Escherichia coli bacA. encoding extremely hydrophobic proteins, were identified in the genomes of Staphylococcus aureus and Streptococcus pneumoniae. Allelic replacement mutagenesis demonstrated that the gene is not essential for in vitro growth in either organism, and the mutants showed no significant changes in growth rate or morphology. The Staph. aureus bacA mutant showed slightly reduced virulence in a mouse model of infection and an eightfold increase in bacitracin susceptibility. However, a Strep. pneumoniae bacA mutant was highly attenuated in a mouse model of infection, and demonstrated an increase in susceptibility to bacitracin of up to 160 000-fold. These observations are consistent with the previously proposed role of BacA protein as undecaprenol kinase.
引用
收藏
页码:1547 / 1553
页数:7
相关论文
共 42 条
  • [1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [2] MECHANISM OF WALL SYNTHESIS IN BACTERIA - ORGANIZATION OF ENZYMES AND ISOPRENOID PHOSPHATES IN MEMBRANE
    ANDERSON, RG
    BADDILEY, J
    HUSSEY, H
    [J]. BIOCHEMICAL JOURNAL, 1972, 127 (01) : 11 - +
  • [3] BADDILEY J, 1973, Biochemical Society Transactions, V1, P1026
  • [4] Effects of different test conditions on MICs of food animal growth-promoting antibacterial agents for enterococci
    Butaye, P
    Devriese, LA
    Haesebrouck, F
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) : 1907 - 1911
  • [5] AMPLIFICATION OF THE BACA GENE CONFERS BACITRACIN RESISTANCE TO ESCHERICHIA-COLI
    CAIN, BD
    NORTON, PJ
    EUBANKS, W
    NICK, HS
    ALLEN, CM
    [J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (12) : 3784 - 3789
  • [6] THE SIGNIFICANCE OF SODIUM CHLORIDE IN STUDIES OF STAPHYLOCOCCI
    CHAPMAN, GH
    [J]. JOURNAL OF BACTERIOLOGY, 1945, 50 (02) : 201 - 203
  • [7] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
    Cole, ST
    Brosch, R
    Parkhill, J
    Garnier, T
    Churcher, C
    Harris, D
    Gordon, SV
    Eiglmeier, K
    Gas, S
    Barry, CE
    Tekaia, F
    Badcock, K
    Basham, D
    Brown, D
    Chillingworth, T
    Connor, R
    Davies, R
    Devlin, K
    Feltwell, T
    Gentles, S
    Hamlin, N
    Holroyd, S
    Hornby, T
    Jagels, K
    Krogh, A
    McLean, J
    Moule, S
    Murphy, L
    Oliver, K
    Osborne, J
    Quail, MA
    Rajandream, MA
    Rogers, J
    Rutter, S
    Seeger, K
    Skelton, J
    Squares, R
    Squares, S
    Sulston, JE
    Taylor, K
    Whitehead, S
    Barrell, BG
    [J]. NATURE, 1998, 393 (6685) : 537 - +
  • [8] SYNERGISM OF THE ANTI-MICROBIAL AGENTS MICONAZOLE, BACITRACIN AND POLYMYXIN-B
    CORNELISSEN, F
    VANDENBOSSCHE, H
    [J]. CHEMOTHERAPY, 1983, 29 (06) : 419 - 427
  • [9] The complete genome of the hyperthermophilic bacterium Aquifex aeolicus
    Deckert, G
    Warren, PV
    Gaasterland, T
    Young, WG
    Lenox, AL
    Graham, DE
    Overbeek, R
    Snead, MA
    Keller, M
    Aujay, M
    Huber, R
    Feldman, RA
    Short, JM
    Olsen, GJ
    Swanson, RV
    [J]. NATURE, 1998, 392 (6674) : 353 - 358
  • [10] EXAFS study of zinc coordination in bacitracin A
    Drablos, F
    Nicholson, DG
    Ronning, M
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1999, 1431 (02): : 433 - 442