Probing single enzyme kinetics in real-time

被引：26

作者：

Chen, Qi ^{[1
]}

Groote, Ramon ^{[1
]}

Schoenherr, Holger ^{[1
]}

Vancso, G. Julius ^{[1
]}

机构：

[1] Univ Twente, Dept Mat Sci & Technol Polymers, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

来源：

CHEMICAL SOCIETY REVIEWS | 2009年 / 38卷 / 09期

关键词：

MOLECULE FLUORESCENCE SPECTROSCOPY; DYNAMIC DISORDER; MOTIONS; MEMORY;

D O I：

10.1039/b903638e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Studies of reaction kinetics at the single enzyme level have become possible due to the swift development of single molecule detection techniques. In this tutorial review, which targets both general and expert research chemists in the biochemical or physical (organic) chemistry field, we briefly introduce some of these fluorescence microscopic techniques and discuss selected studies on single molecule enzymology. The nature of the frequently observed fluctuations in the reaction rate, the so-called dynamic disorder, is described based on various available models.

引用

页码：2671 / 2683

页数：13

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