Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure

被引：39

作者：

Benabou, Sanae ^{[1
]}

Garavis, Miguel ^{[2
]}

Lyonnais, Sebastien ^{[3
]}

Eritja, Ramon ^{[4
]}

Gonzalez, Carlos ^{[2
,5
]}

Gargallo, Raimundo ^{[1
,5
]}

机构：

[1] Univ Barcelona, Dept Analyt Chem, Diagonal 645, E-08028 Barcelona, Spain

[2] CSIC, Inst Phys Chem Rocasolano, Serrano 119, E-28006 Madrid, Spain

[3] Inst Mol Biol Barcelona IBMB CSIC, Baldiri & Reixac 4-8, Barcelona 08028, Spain

[4] CIBER BBN, Inst Adv Chem Catalonia IQAC CSIC, Jordi Girona 18-26, E-08034 Barcelona, Spain

[5] Associated Unit UB CSIC, BIOESTRAN, Barcelona, Spain

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2016年 / 18卷 / 11期

关键词：

MULTIVARIATE CURVE RESOLUTION; MELTING CURVES; G-QUADRUPLEX; RICH STRAND; DNA; PH; THERMODYNAMICS; TEMPERATURE; KINETICS; OLIGODEOXYNUCLEOTIDES;

D O I：

10.1039/c5cp07428b

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The nature and the length of loops connecting cytosine tracts in i-motif structures may affect their stability. In this work, the influence of the nature of the nucleobases located in two of the loops of an intramolecular i-motif is studied using spectroscopy, separation techniques, and multivariate data analysis. The insertion of bases other than thymine induces an additional acid-base equilibrium with pK(a) similar to 4.5. The presence of two guanine bases in the loops, placed opposite to each other, decreases the thermal stability of the structure. In contrast, thymine and cytosine bases in these positions stabilize the structure.

引用

页码：7997 / 8004

页数：8

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