Eukaryotic systems broaden the scope of synthetic biology

被引:30
作者
Haynes, Karmella A. [1 ]
Silver, Pamela A. [1 ]
机构
[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA
基金
美国国家卫生研究院;
关键词
GENE-EXPRESSION; MAMMALIAN-CELLS; ESCHERICHIA-COLI; TRANSGENE EXPRESSION; ENDOPLASMIC-RETICULUM; TRANSCRIPTION FACTORS; SIGNALING PATHWAYS; NATURAL RIBOSWITCH; TOGGLE SWITCH; T-ANTIGEN;
D O I
10.1083/jcb.200908138
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Synthetic biology aims to engineer novel cellular functions by assembling well-characterized molecular parts (i.e., nucleic acids and proteins) into biological "devices" that exhibit predictable behavior. Recently, efforts in eukaryotic synthetic biology have sprung from foundational work in bacteria. Designing synthetic circuits to operate reliably in the context of differentiating and morphologically complex cells presents unique challenges and opportunities for progress in the field. This review surveys recent advances in eukaryotic synthetic biology and describes how synthetic systems can be linked to natural cellular processes in order to manipulate cell behavior and to foster new discoveries in cell biology research.
引用
收藏
页码:589 / 596
页数:8
相关论文
共 79 条
[1]   Rational design of memory in eukaryotic cells [J].
Ajo-Franklin, Caroline M. ;
Drubin, David A. ;
Eskin, Julian A. ;
Gee, Elaine P. S. ;
Landgraf, Dirk ;
Phillips, Ira ;
Silver, Pamela A. .
GENES & DEVELOPMENT, 2007, 21 (18) :2271-2276
[2]   Environmentally controlled invasion of cancer cells by engineered bacteria [J].
Anderson, JC ;
Clarke, EJ ;
Arkin, AP ;
Voigt, CA .
JOURNAL OF MOLECULAR BIOLOGY, 2006, 355 (04) :619-627
[3]   Synthetic biology: new engineering rules for an emerging discipline [J].
Andrianantoandro, Ernesto ;
Basu, Subhayu ;
Karig, David K. ;
Weiss, Ron .
MOLECULAR SYSTEMS BIOLOGY, 2006, 2 (1) :2006.0028
[4]   Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene [J].
Anthony, Jennifer R. ;
Anthony, Larry C. ;
Nowroozi, Farnaz ;
Kwon, Gina ;
Newman, Jack D. ;
Keasling, Jay D. .
METABOLIC ENGINEERING, 2009, 11 (01) :13-19
[5]   A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity [J].
Antunes, Mauricio S. ;
Ha, Suk-Bong ;
Tewari-Singh, Neera ;
Morey, Kevin J. ;
Trofka, Anna M. ;
Kugrens, Paul ;
Deyholos, Michael ;
Medford, June I. .
PLANT BIOTECHNOLOGY JOURNAL, 2006, 4 (06) :605-622
[6]   Engineering key components in a synthetic eukaryotic signal transduction pathway [J].
Antunes, Mauricio S. ;
Morey, Kevin J. ;
Tewari-Singh, Neera ;
Bowen, Tessa A. ;
Smith, J. Jeff ;
Webb, Colleen T. ;
Hellinga, Homme W. ;
Medford, June I. .
MOLECULAR SYSTEMS BIOLOGY, 2009, 5
[7]   Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics [J].
Bashor, Caleb J. ;
Helman, Noah C. ;
Yan, Shude ;
Lim, Wendell A. .
SCIENCE, 2008, 319 (5869) :1539-1543
[8]   Programmable ligand-controlled riboregulators of eukaryotic gene expression [J].
Bayer, TS ;
Smolke, CD .
NATURE BIOTECHNOLOGY, 2005, 23 (03) :337-343
[9]   Positive feedback in eukaryotic gene networks:: cell differentiation by graded to binary response conversion [J].
Becskei, A ;
Séraphin, B ;
Serrano, L .
EMBO JOURNAL, 2001, 20 (10) :2528-2535
[10]   Model-guided design of ligand-regulated RNAi for programmable control of gene expression [J].
Beisel, Chase L. ;
Bayer, Travis S. ;
Hoff, Kevin G. ;
Smolke, Christina D. .
MOLECULAR SYSTEMS BIOLOGY, 2008, 4 (1)