Role of Glutamine Synthetase Isogenes and Herbicide Metabolism in the Mechanism of Resistance to Glufosinate in Lolium perenne L. spp. multiflorum Biotypes from Oregon

被引：51
作者：
Brunharo, Caio A. C. G. [1 ]
Takano, Hudson K. [2 ]
Mallory-Smith, Carol A. [1 ]
Dayan, Franck E. [2 ]
Hanson, Bradley D. [3 ]
机构：
[1] Oregon State Univ, Dept Crop & Soil Sci, 3050 Southwest Campus Way,Crop Sci Bldg, Corvallis, OR 97331 USA
[2] Colorado State Univ, Dept Bioagr Sci & Pest Management, 1777 Campus Delivery, Ft Collins, CO 80523 USA
[3] Univ Calif Davis, Dept Plant Sci, One Shields Ave,MS-4, Davis, CA 95616 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2019年 / 67卷 / 31期
关键词：
enzyme modeling; gene expression; herbicide absorption; herbicide degradation; herbicide translocation; non-target site resistance; phosphinothricin; L-PHOSPHINOTHRICIN GLUFOSINATE; GOOSEGRASS ELEUSINE-INDICA; TARGET-SITE MUTATION; CELLULAR-LOCALIZATION; STRUCTURE VALIDATION; GLYPHOSATE; AMMONIUM; TRANSLOCATION; MOLPROBITY; PROTEIN;
D O I：
10.1021/acs.jafc.9b01392
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Glufosinate-resistant Lolium perenne L. spp. multiflorum biotypes from Oregon exhibited resistance levels up to 2.8-fold the field rate. One resistant biotype (MG) had an amino acid substitution in glutamine synthetase 2 (GS2), whereas the other (OR) exhibited the wild-type genotype. We hypothesized that the amino acid substitution in GS2 is involved in the resistance mechanism in MG and that non-target site resistance mechanisms are present in OR. OR metabolized glufosinate faster than the other two biotypes, with >75% of the herbicide metabolized in comparison to 50% in MG and the susceptible biotype. A mutation in GS2 co-segregating with resistance in MG did not reduce the enzyme activity, with results further supported by our enzyme homology models. This research supports the conclusion that a metabolism mechanism of glufosinate resistance is present in OR and that glufosinate resistance in MG is not due to an altered target site.
引用
收藏
页码：8431 / 8440
页数：10
相关论文
共 53 条
[1]   Gromacs: High performance molecular simulations through multi-level parallelism from laptops to supercomputers [J].
Abraham, Mark James ;
Murtola, Teemu ;
Schulz, Roland ;
Páll, Szilárd ;
Smith, Jeremy C. ;
Hess, Berk ;
Lindah, Erik .
SoftwareX, 2015, 1-2 :19-25
[2]   Target-site mutation associated with glufosinate resistance in Italian ryegrass (Lolium perenne L. ssp multiflorum) [J].
Avila-Garcia, Wilson V. ;
Sanchez-Olguin, Elena ;
Hulting, Andrew G. ;
Mallory-Smith, Carol .
PEST MANAGEMENT SCIENCE, 2012, 68 (09) :1248-1254
[3]   Glyphosate-Resistant Italian Ryegrass (Lolium perenne) Populations also Exhibit Resistance to Glufosinate [J].
Avila-Garcia, Wilson V. ;
Mallory-Smith, Carol .
WEED SCIENCE, 2011, 59 (03) :305-309
[4]   Phloem transport of D,L-glufosinate and acetyl-L-glufosinate in glufosinate-resistant and -susceptible Brassica napus [J].
Beriault, JN ;
Horsman, GP ;
Devine, MD .
PLANT PHYSIOLOGY, 1999, 121 (02) :619-627
[5]   Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.) [J].
Bernard, Stephanie M. ;
Moller, Anders Laurell Blom ;
Dionisio, Giuseppe ;
Kichey, Thomas ;
Jahn, Thomas P. ;
Dubois, Frederic ;
Baudo, Marcela ;
Lopes, Marta S. ;
Terce-Laforgue, Therese ;
Foyer, Christine H. ;
Parry, Martin A. J. ;
Forde, Brian G. ;
Araus, Jose Luis ;
Hirel, Bertrand ;
Schjoerring, Jan K. ;
Habash, Dimah Z. .
PLANT MOLECULAR BIOLOGY, 2008, 67 (1-2) :89-105
[6]   AN ANALYSIS OF TRANSFORMATIONS [J].
BOX, GEP ;
COX, DR .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1964, 26 (02) :211-252
[7]   PHYSICOCHEMICAL ASPECTS OF PHLOEM TRANSLOCATION OF HERBICIDES [J].
BROMILOW, RH ;
CHAMBERLAIN, K ;
EVANS, AA .
WEED SCIENCE, 1990, 38 (03) :305-314
[8]   Multiple Herbicide-Resistant Italian Ryegrass [Lolium perenne L. spp. multiflorum (Lam.) Husnot] in California Perennial Crops: Characterization, Mechanism of Resistance, and Chemical Management [J].
Brunharo, Caio A. C. G. ;
Hanson, Bradley D. .
WEED SCIENCE, 2018, 66 (06) :696-701
[9]   Vacuolar Sequestration of Paraquat Is Involved in the Resistance Mechanism in Lolium perenne L. spp. multiflorum [J].
Brunharo, Caio A. C. G. ;
Hanson, Bradley D. .
FRONTIERS IN PLANT SCIENCE, 2017, 8
[10]   EPSPS duplication and mutation involved in glyphosate resistance in the allotetraploid weed species Poa annua L. [J].
Brunharo, Caio Augusto De Castro Grossi ;
Morran, Sarah ;
Martin, Katie ;
Moretti, Marcelo L. ;
Hanson, Bradley D. .
PEST MANAGEMENT SCIENCE, 2019, 75 (06) :1663-1670
123456