Multicenter Performance Assessment of Carba NP Test

被引：24

作者：

Cunningham, Scott A. ^{[1
]}

Limbago, Brandi ^{[2
]}

Traczewski, Maria ^{[3
]}

Anderson, Karen ^{[2
]}

Hackel, Meredith ^{[4
]}

Hindler, Janet ^{[5
]}

Sahm, Dan ^{[4
]}

Alyanak, Efe

Lawsin, Adrian ^{[2
]}

Gulvik, Christopher A. ^{[2
]}

de Man, Tom J. B. ^{[2
]}

Mandrekar, Jayawant N. ^{[6
]}

Schuetz, Audrey N. ^{[1
,7
]}

Jenkins, Stephen ^{[7
]}

Humphries, Romney ^{[5
]}

Palavecino, Elizabeth ^{[8
]}

Vasoo, Shawn ^{[1
,9
,10
]}

Patel, Robin ^{[1
,10
]}

机构：

[1] Mayo Clin, Div Clin Microbiol, Rochester, MN 55905 USA

[2] Ctr Dis Control & Prevent, Atlanta, GA USA

[3] Clin Microbiol Inst Inc, Wilsonville, OR USA

[4] Int Hlth Management Associates Inc, Schaumburg, IL USA

[5] Univ Calif Los Angeles, Med Ctr, Los Angeles, CA 90024 USA

[6] Mayo Clin, Div Biomed Stat & Informat, Rochester, MN USA

[7] New York Presbyterian Hosp, Weill Cornell Med Coll, New York, NY USA

[8] Wake Forest Baptist Med Ctr, Winston Salem, NC USA

[9] Tan Tock Seng Hosp, Inst Infect Dis & Epidemiol, Singapore, Singapore

[10] Mayo Clin, Div Infect Dis, Rochester, MN 55905 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2017年 / 55卷 / 06期

关键词：

carbapenemase; antimicrobial resistance; screening; Gram-negative bacilli; CARBAPENEMASE-PRODUCING ENTEROBACTERIACEAE; GRAM-NEGATIVE BACILLI; RAPID DETECTION; PSEUDOMONAS-AERUGINOSA; HEMOPHILUS-INFLUENZAE; SCREEN KIT; IDENTIFICATION; CULTURES; PROOFS;

D O I：

10.1128/JCM.00244-17

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Eighty Gram-negative bacilli (54 Enterobacteriaceae and 26 nonfermenting Gram-negative bacilli) obtained from multiple institutions in the United States were distributed in a blinded manner to seven testing laboratories to compare their performance of a test for detection of carbapenemase production, the Carba NP test. The Carba NP test was performed by all laboratories, following the Clinical and Laboratory Standards Institute (CLSI) procedure. Site-versus-site comparisons demonstrated a high level of consistency for the Carba NP assay, with just 3/21 site comparisons yielding a difference in sensitivity (P < 0.05). Previously described limitations with bla(OXA-48-like) carbapenemases and bla(OXA) carbapenemases associated with Acinetobacter baumannii were noted. Based on these data, we demonstrate that the Carba NP test, when implemented with the standardized CLSI methodology, provides reproducible results across multiple sites for detection of carbapenemases.

引用

页码：1954 / 1960

页数：7

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