Map-based cloning of the tomato genomic region that spans the Sw-5 tospovirus resistance gene in tomato

被引：58
作者：
Brommonschenkel, SH [1 ]
Tanksley, SD [1 ]
机构：
[1] CORNELL UNIV,DEPT PLANT BREEDING,ITHACA,NY 14850
来源：
MOLECULAR & GENERAL GENETICS | 1997年 / 256卷 / 02期
关键词：
disease resistance; lycopersicon;
D O I：
10.1007/s004380050553
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two yeast artificial chromosomes (YACs) containing genomic DNA from tomato have been isolated using CT220, an RFLP marker which is tightly linked to the tomato spotted wilt virus resistance gene, Sw-5. High-resolution mapping of the YAC ends and internal YAC probes demonstrated that one of the YAC clones, TY257 (400 kb), spans Sw-5. By chromosome walking in a cosmid library, the position of Sw-5 has been delimited within the YAC to a maximal chromosomal segment of 100 kb, spanned by nine overlapping cosmid clones.
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页码：121 / 126
页数：6
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