Evaluation of the 2-thiobarbituric acid method for the measurement of lipid oxidation in mechanically deboned gamma irradiated chicken meat

This study evaluated the 2-thiobarbituric acid (TBA) method for the measurement of lipid oxidation in samples of mechanically deboned chicken meat (MDCM), both gamma-irradiated with doses of 4.0 and 5.0 kGy and non-irradiated. The values for the percent recuperation of 1, 1,3,3-tetraethoxy-propane (TEP) and for the conversion factor K, for the samples irradiated with doses of 4.0 and 5.0 kGy and the non-irradiated samples, were, respectively, 88.6, 90.0 and 88.6% for the former and 4.98, 4.91 and 4.98 for K. Comparing the results obtained for the samples of MDCM irradiated with doses of 4.0 and 5.0 kGy and the non-irradiated samples, as evaluated by the TBA distillation method, it was observed that the values for percent recuperation of TEP, the values for K and the spectral curves, as also the linear equations of the standard curves for the distillation of malonaldehyde, were similar for all the samples, no specific interference in the TBA test, due to the process of irradiating the MDCM, being detected. (C) 2002 Elsevier Science Ltd. All rights reserved.