Temporal expression patterns of the melatoninergic system in the human thymus of children

被引:6
作者
Cruz-Chamorro, Ivan [1 ,2 ]
Alvarez-Sanchez, Nuria [1 ]
Escalante-Andicoechea, Cristina [1 ]
Carrillo-Vico, Antonio [1 ,2 ]
Rubio, Amalia [1 ,2 ]
Miguel Guerrero, Juan [1 ,2 ,3 ]
Molinero, Patrocinio [1 ,2 ]
Lardone, Patricia J. [1 ,2 ]
机构
[1] Univ Seville, CSIC, Hosp Univ Virgen Rocio, Inst Biomed Sevilla IBiS, Avda Manuel Siurot S-N,Edificio IBiS, Seville 41013, Spain
[2] Univ Seville, Dept Bioquim Med & Biol Mol & Inmunol, Avda Sanchez Pizjuan S-N, E-41009 Seville, Spain
[3] Hosp Univ Virgen Rocio, Dept Bioquim Clin, Avda Manuel Siurot S-N, Seville 41013, Spain
来源
MOLECULAR METABOLISM | 2019年 / 28卷
关键词
Melatonin; Thymus; AANAT; ASMT; Melatonin receptor; Nuclear receptor ROR-alpha; AGE-RELATED-CHANGES; RECEPTOR EXPRESSION; HUMAN-LYMPHOCYTES; PINEAL-GLAND; MEMBRANE; RAT; MT1; BIOSYNTHESIS; TISSUES; PROTEIN;
D O I
10.1016/j.molmet.2019.07.007
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objectives: To obtain greater knowledge of the extra-pineal sources of melatonin during development, the amount of indolamine and the expression levels of the last two enzymes involved in its biosynthesis, Arylalkylamine N-acetyltransferase (AANAT) and acetylserotonin O-methyltransferase (ASMT), were analyzed in the human thymus from children from three different age groups (from days to years). The melatonin membrane and nuclear receptor expression levels also were studied. Methods: Quantitative reverse transcriptase PCR and western blot were performed to investigate the receptor and enzyme expression levels. The results were examined and correlated with the ages of the thymuses. Results: We found high levels of indolamine in the thymuses of newborns (younger than 1 month), which decreased during development; thymuses from the months (from 2 to 11 months) and years (from 1 to 12 years) groups showed lower levels. A similar decline was also observed in the mRNA of the AANAT enzyme and the expression levels of melatonin receptors. However, ASMT expression was exactly the opposite, with low levels in the newborn group and higher levels in the years group. Our results show that the thymic synthesis of melatonin occurs very early in childhood. Additionally, this is the first report that is focused on melatonin receptors expression in the human thymus. Conclusion: Considering the limited melatonin synthesis performed by the newborn pineal gland, we suggest that the high levels of melatonin found in human thymus in this experimental group arise from synthesis in the tissue itself, which could be contributing to the immune efficiency at the thymic level. (C) 2019 The Authors. Published by Elsevier GmbH.
引用
收藏
页码:83 / 90
页数:8
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