Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts

被引：7

作者：

Bouzahzah, Boumediene ^{[1
,2
]}

Yurchenko, Vyacheslav ^{[1
,2
]}

Nagajyothi, Fnu ^{[1
,2
]}

Hulit, James ^{[1
,2
]}

Sadofsky, Moshe ^{[1
,2
]}

Braunstein, Vicki L. ^{[1
,2
]}

Mukherjee, Shankar ^{[1
,2
]}

Weiss, Hannah ^{[1
,2
]}

Machado, Fabiana S. ^{[3
,4
]}

Pestell, Richard G. ^{[5
,6
]}

Lisanti, Michael P. ^{[5
,6
,7
,8
]}

Tanowitz, Herbert B. ^{[1
,2
]}

Albanese, Chris ^{[9
,10
]}

机构：

[1] Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA

[3] Cincinnati Childrens Hosp Med Ctr, Div Mol Immunol, Cincinnati, OH USA

[4] Univ Cincinnati, Coll Med, Cincinnati, OH USA

[5] Thomas Jefferson Univ, Dept Canc Biol, Philadelphia, PA 19107 USA

[6] Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA 19107 USA

[7] Univ Genoa, Muscular Neurodegenerat Dis Unit, Genoa, Italy

[8] G Gaslini Pediat Inst, Genoa, Italy

[9] Georgetown Univ, Med Ctr, Dept Oncol, Washington, DC 20007 USA

[10] Georgetown Univ, Med Ctr, Lombardi Comprehens Canc Ctr, Washington, DC 20007 USA

来源：

CELL CYCLE | 2008年 / 7卷 / 04期

关键词：

Trypanosoma cruzi; Chagas disease; cell cycle regulatory proteins; cyclin D1; cyclin D1 promoter-luciferase (CD1Luc) reporters;

D O I：

10.4161/cc.7.4.5327

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L6E9 myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.

引用

页码：500 / 503

页数：4

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