Mutasynthesis of rapamycin analogues through the manipulation of a gene governing starter unit biosynthesis

被引：81
作者：
Gregory, MA
Petkovic, H
Lill, RE
Moss, SJ
Wilkinson, B
Gaisser, S
Leadlay, PF
Sheridan, RM
机构：
[1] Biot Technol Ltd, Saffron Walden CB10 1XL, Essex, England
[2] Dept Biochem, Cambridge CB2 1GA, England
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 30期
基金：
英国生物技术与生命科学研究理事会;
关键词：
biosynthesis; macrocycles; mutasynthesis; natural products; polyketides;
D O I：
10.1002/anie.200462784
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Chemical Equation Presented) The surprising discovery has been made that the gene product RapK is required for production of the starter unit in the rapamycin polyketide cluster. It is shown that a deletion mutant (Streptomyces hygroscopicus MG2-10) provides a clean background for the mutasynthesis of pre-rapamycin (the precursor to rapamycin, see structure) when fed with novel starter units. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：4757 / 4760
页数：4
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