ION-EXCHANGE CHROMATOGRAPHY

被引：70

作者：

Jungbauer, Alois ^{[1
]}

Hahn, Rainer ^{[1
]}

机构：

[1] Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Vienna, Austria

来源：

GUIDE TO PROTEIN PURIFICATION, SECOND EDITION | 2009年 / 463卷

关键词：

SEPARATION; COLUMNS; RETENTION; MONOLITHS; MEDIA; MODEL;

D O I：

10.1016/S0076-6879(09)63022-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic toot and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can be obtained. In this chapter, the guidance will be provided for binding and elution conditions and selection of stationary phases.

引用

页码：349 / 371

页数：23

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