ION-EXCHANGE CHROMATOGRAPHY

被引：70

作者：

Jungbauer, Alois ^{[1
]}

Hahn, Rainer ^{[1
]}

机构：

[1] Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Vienna, Austria

来源：

GUIDE TO PROTEIN PURIFICATION, SECOND EDITION | 2009年 / 463卷

关键词：

SEPARATION; COLUMNS; RETENTION; MONOLITHS; MEDIA; MODEL;

D O I：

10.1016/S0076-6879(09)63022-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic toot and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can be obtained. In this chapter, the guidance will be provided for binding and elution conditions and selection of stationary phases.

引用

页码：349 / 371

页数：23

共 50 条

[1] ION-EXCHANGE CHROMATOGRAPHY - REACTIVE ION-EXCHANGE
JANAUER, GE
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1976, : 63 - 63
[2] ION-EXCHANGE CHROMATOGRAPHY
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METHODS IN ENZYMOLOGY, 1990, 182 : 309 - 317
[3] ION-EXCHANGE CHROMATOGRAPHY
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ANALYTICA CHIMICA ACTA, 1967, 38 (1-2) : 163 - +
[4] CHROMATOGRAPHY AND ION-EXCHANGE
不详
NATURE, 1949, 163 (4137) : 235 - 237
[5] APPARATUS FOR ION-EXCHANGE CHROMATOGRAPHY
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BIOCHEMICAL JOURNAL, 1957, 67 : P33 - P33
[6] ION-EXCHANGE CHROMATOGRAPHY IN ALCOHOL
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NATURE, 1955, 176 (4478) : 398 - 400
[7] ELECTROMODULATED ION-EXCHANGE CHROMATOGRAPHY
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ANALYTICAL CHEMISTRY, 1986, 58 (07) : 1574 - 1575
[8] ISOELECTRIC ION-EXCHANGE CHROMATOGRAPHY
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JOURNAL OF THE AMERICAN OSTEOPATHIC ASSOCIATION, 1978, 78 (02): : 142 - 142
[9] SUPERFICIAL ION-EXCHANGE CHROMATOGRAPHY
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[10] DEVELOPMENTS IN ION-EXCHANGE CHROMATOGRAPHY
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