Expression profiles of long noncoding RNAs in cutaneous squamous cell carcinoma

被引：31

作者：

Sand, Michael ^{[1
,2
]}

Bechara, Falk G. ^{[1
]}

Sand, Daniel ^{[3
]}

Gambichler, Thilo ^{[1
]}

Hahn, Stephan A. ^{[4
]}

Bromba, Michael ^{[2
]}

Stockfleth, Eggert ^{[1
]}

Hessam, Schapoor ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Dept Dermatol Venereol & Allergol, Dermatol Surg Unit, D-44791 Bochum, Germany

[2] Catholic Clin Ruhr Peninsula, St Josef Hosp, Dept Plast Surg, D-45257 Essen, Germany

[3] Univ Michigan, Kellogg Eye Ctr, Ann Arbor, MI 48105 USA

[4] Knappschaftskrankenhaus Univ Bochum, Dept Internal Med, Zentrum Klin Forsch, Labor Mol Gastroenterol Onkol, D-44780 Bochum, Germany

来源：

EPIGENOMICS | 2016年 / 8卷 / 04期

关键词：

cutaneous squamous cell carcinoma; epithelial skin cancer; long noncoding RNAs; noncoding RNAs; nonmelanoma skin cancer; POTENTIAL ROLE; CANCER; HOTAIR; MICRORNA; REVEALS; LNCRNAS; DIFFERENTIATION; HOMEOSTASIS; ANNOTATION; DATABASE;

D O I：

10.2217/epi-2015-0012

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: Despite there being over 35,000 different long noncoding RNA (lncRNA) sequences described little is known regarding their molecular-pathological role in cutaneous squamous cell carcinoma (cSCC). Materials & methods: In this pilot study, lncRNA and mRNA expression profiles were determined in cSCC and control (n = 6) by an Arraystar human lncRNA Microarray. Kyoto Encyclopedia of Genes and Genomes pathway enrichment and gene ontology analysis of mRNAs was performed. Results: Analysis of differential expression revealed 1516 upregulated lncRNAs and 2586 downregulated lncRNAs in cSCC compared with controls. Data analysis identified known oncogenic lncRNAs, such as the HOX transcript antisense RNA HOTAIR, among the differentially expressed lncRNA sequences. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that focal adhesion, extracellular matrix and the oncogenic phosphatidylinositol 3'-kinase-Akt signaling pathway had the highest enrichment scores. Conclusion: This study provides the first evidence for differential expression of lncRNA in cSCC and serves as a template for further, larger functional in-depth analyses regarding cSCC molecular lncRNAs.

引用

页码：501 / 518

页数：18

共 73 条

[1] lncRNAdb: a reference database for long noncoding RNAs [J].

Amaral, Paulo P. ;

Clark, Michael B. ;

Gascoigne, Dennis K. ;

Dinger, Marcel E. ;

Mattick, John S. .

NUCLEIC ACIDS RESEARCH, 2011, 39 :D146-D151

[2]

[Anonymous], COLD SPRING HARB PER

[3] Ultraconserved elements in the human genome [J].

Bejerano, G ;

Pheasant, M ;

Makunin, I ;

Stephen, S ;

Kent, WJ ;

Mattick, JS ;

Haussler, D .

SCIENCE, 2004, 304 (5675) :1321-1325

[4] Controlling the false discovery rate in behavior genetics research [J].

Benjamini, Y ;

Drai, D ;

Elmer, G ;

Kafkafi, N ;

Golani, I .

BEHAVIOURAL BRAIN RESEARCH, 2001, 125 (1-2) :279-284

[5] Single-nucleotide polymorphisms in pigment genes and nonmelanoma skin cancer predisposition: a systematic review [J].

Binstock, M. ;

Hafeez, F. ;

Metchnikoff, C. ;

Arron, S. T. .

BRITISH JOURNAL OF DERMATOLOGY, 2014, 171 (04) :713-721

[6] Inhibition of Epidermal Growth Factor Receptor and PI3K/Akt Signaling Suppresses Cell Proliferation and Survival through Regulation of Stat3 Activation in Human Cutaneous Squamous Cell Carcinoma [J].

Bito, Toshinori ;

Sumita, Nahoko ;

Ashida, Masashi ;

Budiyanto, Arief ;

Ueda, Masato ;

Ichihashi, Masamitsu ;

Tokura, Yoshiki ;

Nishigori, Chikako .

JOURNAL OF SKIN CANCER, 2011, 2011

[7] Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses [J].

Cabili, Moran N. ;

Trapnell, Cole ;

Goff, Loyal ;

Koziol, Magdalena ;

Tazon-Vega, Barbara ;

Regev, Aviv ;

Rinn, John L. .

GENES & DEVELOPMENT, 2011, 25 (18) :1915-1927

[8] Targeting transcription factor STAT3 for cancer prevention and therapy [J].

Chai, Edna Zhi Pei ;

Shanmugam, Muthu K. ;

Arfuso, Frank ;

Dharmarajan, Arunasalam ;

Wang, Chao ;

Kumar, Alan Prem ;

Samy, Ramar Perumal ;

Lim, Lina H. K. ;

Wang, Lingzhi ;

Goh, Boon Cher ;

Ahn, Kwang Seok ;

Hui, Kam Man ;

Sethi, Gautam .

PHARMACOLOGY & THERAPEUTICS, 2016, 162 :86-97

[9]

Consortium TGO, 2015, Nucleic Acids Res, V43, pD1049

[10] Treatment of Unresectable and Metastatic Cutaneous Squamous Cell Carcinoma [J].

Cranmer, Lee D. ;

Engelhardt, Candace ;

Morgan, Sherif S. .

ONCOLOGIST, 2010, 15 (12) :1320-1328

← 1 2 3 4 5 6 7 8 →