Evidence that microRNAs are associated with translating messenger RNAs in human cells

被引：226
作者：
Maroney, Patricia A.
Yu, Yang
Fisher, Jesse
Nilsen, Timothy W.
机构：
[1] Case Western Reserve Univ, Ctr RNA Mol Biol, Sch Med, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Dept Biochem, Sch Med, Cleveland, OH 44106 USA
来源：
NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2006年 / 13卷 / 12期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1038/nsmb1174
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs (miRNAs) regulate gene expression post-transcriptionally by binding the 3' untranslated regions of target mRNAs. We examined the subcellular distribution of three miRNAs in exponentially growing HeLa cells and found that the vast majority are associated with mRNAs in polysomes. Several lines of evidence indicate that most of these mRNAs, including a known miRNA-regulated target (KRAS mRNA), are actively being translated.
引用
收藏
页码：1102 / 1107
页数：6
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