Targeted chromosome elimination from ES-somatic hybrid cells

被引：66
作者：
Matsumura, Hiroyuki
Tada, Masako
Otsuji, Tomomi
Yasuchika, Kentaro
Nakatsuji, Norio
Surani, Azim
Tada, Takashi
机构：
[1] Kyoto Univ, Inst Frontier Med Sci, Sakyo Ku, Kyoto 6068507, Japan
[2] ReproCELL Inc, Chiyoda Ku, Tokyo 1000011, Japan
[3] Univ Cambridge, Wellcome Trust Canc Res UK Gurdon Inst Canc & Dev, Cambridge CB2 1QN, England
[4] CREST, JST, Kawaguchi, Saitama 3320012, Japan
来源：
NATURE METHODS | 2007年 / 4卷 / 01期
关键词：
D O I：
10.1038/NMETH973
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.
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页码：23 / 25
页数：3
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