Validation of a direct time-resolved fluoroimmunoassay for progesterone in milk from dairy and beef cows

被引：14

作者：

Oku, Yoko ^{[1
]}

Osawa, Takeshi ^{[1
]}

Hirata, Toh-Ichi ^{[2
]}

Kon, Naoki ^{[2
]}

Akasaka, Shigeru ^{[2
]}

Senosy, Waleed S. ^{[1
,3
]}

Takahashi, Toru ^{[4
]}

Izaike, Yoshiaki ^{[1
]}

机构：

[1] Iwate Univ, Lab Theriogenol, Dept Vet Med, Fac Agr, Morioka, Iwate 0208550, Japan

[2] Iwate Univ, Field Sci Ctr, Omyojin Expt Stn, Fac Agr, Morioka, Iwate 0208550, Japan

[3] Assiut Univ, Dept Theriogenol, Fac Vet Med, Assiut 71526, Egypt

[4] Natl Inst Agrobiol Sci, Reprod Biol Res Unit, Tsukuba, Ibaraki 3058602, Japan

来源：

VETERINARY JOURNAL | 2011年 / 190卷 / 02期

关键词：

Beef cow; Dairy cow; Hormone assay; Milk; Progesterone; TR-FIA; BOVINE-MILK; PLASMA; CATTLE; BLOOD; SERUM;

D O I：

10.1016/j.tvjl.2010.10.024

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this study was to validate a direct time-resolved fluoroimmunoassay (TR-FIA) for quantifying progesterone concentrations in milk during the bovine oestrous cycle. Holstein-Friesian and suckled and non-suckled Japanese Black cows were used to demonstrate the relationship between milk and plasma progesterone concentrations and to monitor progesterone profiles in milk and plasma during the oestrous cycle. The minimum detection level of the assay was 1.53 ng/mL Progesterone concentrations in milk and plasma changed in a similar manner throughout the oestrous cycle in dairy and beef cows, and milk and plasma progesterone profiles were significantly correlated (P < 0.001). The study confirmed that a direct TR-FIA can be used to monitor the oestrous cycle in cattle and to quantify progesterone concentrations in whole milk. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：244 / 248

页数：5

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