Physical exercise increases ROCK activity in the skeletal muscle of middle-aged rats

被引：8

作者：

Munoz, Vitor Rosetto ^{[1
]}

Gaspar, Rafael Calais ^{[1
]}

Esteca, Marcos Vinicius ^{[2
]}

Baptista, Igor Luchini ^{[2
]}

Lins Vieira, Renan Fudoli ^{[1
]}

Ramos da Silva, Adelino Sanchez ^{[3
,4
]}

de Moura, Leandro Pereira ^{[1
,5
]}

Cintra, Dennys Esper ^{[4
,6
]}

Ropelle, Eduardo Rochete ^{[1
,5
]}

Pauli, Jose Rodrigo ^{[1
,5
]}

机构：

[1] Univ Estadual Campinas, UNICAMP, Lab Mol Biol Exercise, Limeira, SP, Brazil

[2] Univ Estadual Campinas, UNICAMP, Lab Cellular & Tissue Biol, Fac Appl Sci, Limeira, Brazil

[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP, Brazil

[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP, Brazil

[5] Univ Estadual Campinas, UNICAMP, OCRC, Campinas, SP, Brazil

[6] Univ Estadual Campinas, UNICAMP, Lab Nutr Genom, Limeira, SP, Brazil

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 2020年 / 186卷

基金：

巴西圣保罗研究基金会;

关键词：

Aging; Physical exercise; Insulin resistance; Rho-kinase (ROCK); GLUCOSE-TRANSPORT; INSULIN-RESISTANCE; RHO-KINASE; PROTEINS;

D O I：

10.1016/j.mad.2020.111213

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The physical exercise is a potential strategy to control age-related metabolic disorders, such as insulin resistance, impaired glucose homeostasis, and type 2 diabetes. Rho-kinase (ROCK) increases skeletal muscle glucose uptake through Insulin Receptor Substrate 1 (IRS1) phosphorylation. Here, we investigated the role of physical exercise in ROCK pathway in the skeletal muscle of Fischer middle-aged rats. Firstly, we observed the ROCK distribution in different skeletal muscle fiber types. ROCK signaling pathway (ROCK1 and ROCK2) and activity (pMYPT1) were higher in the soleus, which was associated with increased insulin signaling pathway (pIR, pIRS1, pPDK, pGSK3 beta). Middle-aged rats submitted to physical exercise, showed the upregulation of ROCK2 content and normalized RhoA (ROCK activator enzyme) levels in soleus muscle compared with middle-aged sedentary rats. These molecular changes in middle-aged exercised rats were accompanied by higher insulin signaling (pIRS1, pGSK3 beta, pAS160, GLUT4) in the soleus muscle. Reinforcing these findings, when pharmacological inhibition of ROCK activity was performed (using Y-27632), the insulin signaling pathway and glucose metabolism-related genes (Tpi, Pgk1, Pgam2, Eno3) were decreased in the soleus muscle of exercised rats. In summary, ROCK signaling seems to contribute with whole-body glucose homeostasis (similar to 50 %) through its higher upregulation in the soleus muscle in middle-aged exercised rats.

引用

页数：8

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