Population structure and uropathogenic virulence-associated genes of faecal Escherichia coli from healthy young and elderly adults

被引:29
作者
Vollmerhausen, Tara L. [1 ]
Ramos, Nubia L. [1 ]
Guendogdu, Aycan [1 ]
Robinson, Wayne [2 ]
Brauner, Annelie [3 ]
Katouli, Mohammad [1 ]
机构
[1] Univ Sunshine Coast, Fac Sci Hlth & Educ, Maroochydore, Qld 4558, Australia
[2] Charles Sturt Univ, Sch Environm Sci, Thurgoona, NSW 2641, Australia
[3] Karolinska Univ Hosp, Dept Clin Microbiol, MTC, S-17176 Stockholm, Sweden
关键词
URINARY-TRACT-INFECTION; PHYLOGENETIC GROUP; ACUTE CYSTITIS; RISK-FACTORS; STRAINS; WOMEN; EPIDEMIOLOGY; DIVERSITY; STOOLS; COSTS;
D O I
10.1099/jmm.0.027037-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We investigated the population structures of faecal Escherichia coli in 30 healthy young adults (13 males and 17 females) aged between 20 and 45 years and 29 elderly adults (14 females and 15 males) aged between 65 and 77 years. In all, 1566 strains were typed with the PhPlate system and grouped into biochemical phenotypes (BPTs). Strains with shared BPTs were further typed using randomly amplified polymorphic DNA analysis. Forty-four per cent of the strains were shared between two or more age and gender groups. Elders had a significantly higher (P<0.001) number of BPTs (mean +/- standard error 3.3 +/- 0.27) than younger groups (1.82 +/- 0.27). Phylogenetic affiliation and virulence-associated genes (VAGs) of the strains showed that more than 80 % of the strains belonging to dominant types belonged to phylogroups B2 and D. Amongst dominant BPTs, phylogenetic group A was significantly associated with females (P<0.0001), and elders were more likely to carry group D (P<0.0124). Elderly males had a higher prevalence of VAGs than young males (P<0.0001) and young females (P<0.0005). We conclude that there is a lower prevalence of E. coli with uropathogenic properties in healthy young adults than in elders.
引用
收藏
页码:574 / 581
页数:8
相关论文
共 38 条
[1]   Type 1 fimbriated Escherichia coli-mast cell interactions in cystitis [J].
Abraham, SN ;
Shin, JS ;
Malaviya, R .
JOURNAL OF INFECTIOUS DISEASES, 2001, 183 :S51-S55
[2]   Clonal groups of enteropathogenic Escherichia coli isolated in case-control studies of diarrhoea in Bangladesh [J].
Ansaruzzaman, M ;
Albert, MJ ;
Nahar, S ;
Byun, R ;
Katouli, M ;
Kühn, I ;
Möllby, R .
JOURNAL OF MEDICAL MICROBIOLOGY, 2000, 49 (02) :177-185
[3]   MOLECULAR EPIDEMIOLOGY OF ADHESIN AND HEMOLYSIN VIRULENCE FACTORS AMONG UROPATHOGENIC ESCHERICHIA-COLI [J].
ARTHUR, M ;
JOHNSON, CE ;
RUBIN, RH ;
ARBEIT, RD ;
CAMPANELLI, C ;
KIM, C ;
STEINBACH, S ;
AGARWAL, M ;
WILKINSON, R ;
GOLDSTEIN, R .
INFECTION AND IMMUNITY, 1989, 57 (02) :303-313
[4]   Chromosomal regions specific to pathogenic isolates of Escherichia coli have a phylogenetically clustered distribution [J].
Boyd, EF ;
Hartl, DL .
JOURNAL OF BACTERIOLOGY, 1998, 180 (05) :1159-1165
[5]   PCR differentiation of Escherichia coli from other Gram-negative bacteria using primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein [J].
Chen, J ;
Griffiths, MW .
LETTERS IN APPLIED MICROBIOLOGY, 1998, 27 (06) :369-371
[6]   Rapid and simple determination of the Escherichia coli phylogenetic group [J].
Clermont, O ;
Bonacorsi, S ;
Bingen, E .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4555-4558
[7]   Prospective Cohort Study of Microbial and Inflammatory Events Immediately Preceding Escherichia coli Recurrent Urinary Tract Infection in Women [J].
Czaja, C. A. ;
Stamm, W. E. ;
Stapleton, A. E. ;
Roberts, P. L. ;
Hawn, T. R. ;
Scholes, D. ;
Samadpour, M. ;
Hultgren, S. J. ;
Hooton, T. M. .
JOURNAL OF INFECTIOUS DISEASES, 2009, 200 (04) :528-536
[8]   Pathogenicity-Associated Islands in Extraintestinal Pathogenic Escherichia coli Are Fitness Elements Involved in Intestinal Colonization [J].
Diard, Mederic ;
Garry, Louis ;
Selva, Marjorie ;
Mosser, Thomas ;
Denamur, Erick ;
Matic, Ivan .
JOURNAL OF BACTERIOLOGY, 2010, 192 (19) :4885-4893
[9]   Commensal Escherichia coli isolates are phylogenetically distributed among geographically distinct human populations [J].
Duriez, P ;
Clermont, O ;
Bonacorsi, S ;
Bingen, E ;
Chaventre, A ;
Elion, J ;
Picard, B ;
Denamur, E .
MICROBIOLOGY-SGM, 2001, 147 :1671-1676
[10]   Transmission of uropathogens between sex partners [J].
Foxman, B ;
Zhang, LX ;
Tallman, P ;
Andree, BC ;
Geiger, AM ;
Koopman, JS ;
Gillespie, BW ;
Palin, KA ;
Sobel, JD ;
Rode, CK ;
Bloch, CA ;
Marrs, CF .
JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (04) :989-992