Localization of Multiple O-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation

被引：16

作者：

Baba, Takashi ^{[2
]}

Zhang, Zoe ^{[1
]}

Liu, Suya ^{[2
]}

Burton, Lyle ^{[2
]}

Ryumin, Pavel ^{[2
]}

Le Blanc, J. C. Yves ^{[2
]}

机构：

[1] SCIEX Ltd, Redwood City, CA 94065 USA

[2] SCIEX Ltd, Concord, ON L4K 4V8, Canada

来源：

JOURNAL OF PROTEOME RESEARCH | 2022年 / 21卷 / 10期

关键词：

electron-activated dissociation; hot electron capture dissociation; mass spectrometry; O-linked glycopeptides; fetuin; time-of-flight mass spectrometer; scheduled high-resolution multiple reaction monitoring; Zeno trap pulsing; MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; GLYCOSYLATION ANALYSIS; PEPTIDE; ETHCD;

D O I：

10.1021/acs.jproteome.2c00378

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method to obtain a comprehensive profile of multiple glycosylations in glycopeptide isoforms. We detected a wide range of abundances of various O-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry. To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows. In addition, Zeno trap pulsing was activated to enhance the sensitivity of the time-of-flight mass spectrometer. Sixty-nine O-glycopeptides of the long O- glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.

引用

收藏

页码：2462 / 2471

页数：10

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