Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3′-extension of 5S rRNA in Saccharomyces cerevisiae

被引：31

作者：

Nariai, M ^{[1
]}

Tanaka, T ^{[1
]}

Okada, T ^{[1
]}

Shirai, C ^{[1
]}

Horigome, C ^{[1
]}

Mizuta, K ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biosphere Sci, Dept Bioresource Sci & Technol, Higashihiroshima 7398528, Japan

来源：

NUCLEIC ACIDS RESEARCH | 2005年 / 33卷 / 14期

基金：

日本学术振兴会;

关键词：

D O I：

10.1093/nar/gki772

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rrs1p, a ribosomal protein L11-binding protein, has an essential role in biogenesis of 60S ribosomal subunits. We obtained conditionally synthetic lethal allele with the rrs1-5 mutation and determined that the mutation is in REX1, which encodes an exonuclease. The highly conserved leucine at 305 was substituted with tryptophan in rex1-1. The rex1-1 allele resulted in 3'-extended 5S rRNA. Polysome analysis revealed that rex1-1 and rrs1-5 caused a synergistic defect in the assembly of 60S ribosomal subunits. In vivo and in vitro binding assays indicate that Rrs1p interacts with the ribosomal protein L5-5S rRNA complex. The rrs1-5 mutation weakens the interaction between Rrs1p with both L5 and L11. These data suggest that the assembly of L5-5S rRNA on 60S ribosomal subunits coordinates with assembly of L11 via Rrs1p.

引用

页码：4553 / 4562

页数：10

共 32 条

[1]

[Anonymous], 1994, METHODS YEAST GENETI

[2] Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway [J].