"Molecular Activity Painting": Switch-like, Light-Controlled Perturbations inside Living Cells

被引：36

作者：

Chen, Xi ^{[1
]}

Venkatachalapathy, Muthukumaran ^{[2
,3
]}

Kamps, Dominic ^{[2
,3
]}

Weigel, Simone ^{[4
]}

Kumar, Ravi ^{[5
,6
]}

Orlich, Michael ^{[2
,3
]}

Garrecht, Ruben ^{[4
]}

Hirtz, Michael ^{[5
,6
]}

Niemeyer, Christof M. ^{[4
]}

Wu, Yao-Wen ^{[1
]}

Dehmelt, Leif ^{[2
,3
]}

机构：

[1] Max Planck Gesell, Chem Genom Ctr, Dortmund, Germany

[2] Tech Univ Dortmund, Dept System Cell Biol, Max Planck Inst Mol Physiol, Dortmund, Germany

[3] Tech Univ Dortmund, Fak Chem & Chem Biol, Dortmund, Germany

[4] Karlsruhe Inst Technol, Inst Biol Interfaces, Karlsruhe, Germany

[5] Karlsruhe Inst Technol, Inst Nanotechnol INT, Karlsruhe, Germany

[6] Karlsruhe Inst Technol, Karlsruhe Nano Micro Facil, Karlsruhe, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2017年 / 56卷 / 21期

基金：

欧洲研究理事会;

关键词：

immobilization; optochemical biology; photochemically induced dimerization; Rho GTPases; signal transduction; PROTEIN-PROTEIN INTERACTIONS; SPATIOTEMPORAL CONTROL; MEDIATED INDUCTION; MAMMALIAN-CELLS; PLASMA-MEMBRANE; LIVE CELLS; DIMERIZATION; DIFFUSION; MIGRATION; BIOLOGY;

D O I：

10.1002/anie.201611432

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging owing to the lateral diffusion of plasma membrane targeted molecules. Herein we present "molecular activity painting" (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface-immobilized antibodies blocks lateral diffusion, enabling rapid and stable "painting" of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA-myosin activator GEF-H1 induces patterned acto-myosin contraction inside living cells.

引用

页码：5916 / 5920

页数：5

共 33 条

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