Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

被引：5

作者：

Xiao, Yixin ^{[1
,2
]}

Yang, Fan ^{[1
,2
]}

Liu, Fumin ^{[1
,2
]}

Yao, Hangping ^{[1
,2
]}

Wu, Nanping ^{[1
,2
]}

Wu, Haibo ^{[1
,2
]}

机构：

[1] Zhejiang Univ, State Key Lab Diag & Treatment Infect Dis, 79 Qingchun Rd, Hangzhou 310003, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Natl Clin Res Ctr Infect Dis, 79 Qingchun Rd, Hangzhou 310003, Peoples R China

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2021年 / 33卷 / 03期

关键词：

avian influenza A virus; H2; subtype; real-time RT-PCR;

D O I：

10.1177/1040638721994810

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

引用

页码：577 / 581

页数：5

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