Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

被引：5

作者：

Xiao, Yixin ^{[1
,2
]}

Yang, Fan ^{[1
,2
]}

Liu, Fumin ^{[1
,2
]}

Yao, Hangping ^{[1
,2
]}

Wu, Nanping ^{[1
,2
]}

Wu, Haibo ^{[1
,2
]}

机构：

[1] Zhejiang Univ, State Key Lab Diag & Treatment Infect Dis, 79 Qingchun Rd, Hangzhou 310003, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Natl Clin Res Ctr Infect Dis, 79 Qingchun Rd, Hangzhou 310003, Peoples R China

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2021年 / 33卷 / 03期

关键词：

avian influenza A virus; H2; subtype; real-time RT-PCR;

D O I：

10.1177/1040638721994810

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

引用

收藏

页码：577 / 581

页数：5

相关论文

共 22 条

[1] Rapid molecular haemagglutinin subtyping of avian influenza isolates by specific real-time. RT-PCR tests [J].

Elizalde, Maia ;

Agueero, Montserrat ;

Buitrago, Dolores ;

Yuste, Maria ;

Luisa Arias, Maria ;

Jesus Munoz, Maria ;

Lelli, Davide ;

Perez-Ramirez, Elisa ;

Moreno-Martin, Ana Maria ;

Fernandez-Pinero, Jovita .

JOURNAL OF VIROLOGICAL METHODS, 2014, 196 :71-81

[2] A Systematic Review and Meta-Analysis of Practices Exposing Humans to Avian Influenza Viruses, Their Prevalence, and Rationale [J].

Fournie, Guillaume ;

Hog, Erling ;

Barnett, Tony ;

Pfeiffer, Dirk U. ;

Mangtani, Punam .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2017, 97 (02) :376-388

[3]

He PY, 2017, ANN CLIN LAB SCI, V47, P178

[4] Identification and Characterization of H2N3 Avian Influenza Virus from Backyard Poultry and Comparison with Novel H2N3 Swine Influenza Virus [J].

Killian, Mary Lea ;

Zhang, Yan ;

Panigrahy, Brundaban ;

Trampel, Darrell ;

Yoon, Kyoung-Jin .

AVIAN DISEASES, 2011, 55 (04) :611-619

[5] H2N5 influenza virus isolates from terns in Australia: genetic reassortants between those of the Eurasian and American lineages [J].

Kishida, Noriko ;

Sakoda, Yoshihiro ;

Shiromoto, Mai ;

Bai, Gui-Rong ;

Isoda, Norikazu ;

Takada, Ayato ;

Laver, Graeme ;

Kida, Hiroshi .

VIRUS GENES, 2008, 37 (01) :16-21

[6] Development of a realtime RT-PCR assay for the rapid detection of influenza A(H2) viruses [J].

Komissarov, Andrey ;

Fadeev, Artem ;

Kosheleva, Anna ;

Sintsova, Kseniya ;

Grudinin, Mikhail .

MOLECULAR AND CELLULAR PROBES, 2017, 35 :57-63

[7] Influenza A/B virus detection and influenza A virus subtyping with emphasis on the novel H7N9 virus by using multiplex real-time RT-PCR [J].

Kuo, Rei-Lin ;

Yang, Shu-Li ;

Liu, Yi-Chun ;

Chen, Lu-Ting ;

Mok, Chee-Keng ;

Kuo, Shu-Ming ;

Shih, Shin-Ru ;

Tsao, Kuo-Chien .

JOURNAL OF VIROLOGICAL METHODS, 2014, 208 :41-46

[8] The influenza virus gene pool in a poultry market in South Central China [J].

Liu, M ;

He, SQ ;

Walker, D ;

Zhou, NN ;

Perez, DR ;

Mo, B ;

Li, F ;

Huang, XT ;

Webster, RG ;

Webby, RJ .

VIROLOGY, 2003, 305 (02) :267-275

[9] Next generation sequencing for whole genome analysis and surveillance of influenza A viruses [J].

McGinnis, Jana ;

Laplante, Jennifer ;

Shudt, Matthew ;

St George, Kirsten .

JOURNAL OF CLINICAL VIROLOGY, 2016, 79 :44-50

[10] Vaccinate for the next H2N2 pandemic now [J].

Nabel, Gary J. ;

Wei, Chih-Jen ;

Ledgerwood, Julie E. .

NATURE, 2011, 471 (7337) :157-158