Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

被引：5
作者：
Xiao, Yixin [1 ,2 ]
Yang, Fan [1 ,2 ]
Liu, Fumin [1 ,2 ]
Yao, Hangping [1 ,2 ]
Wu, Nanping [1 ,2 ]
Wu, Haibo [1 ,2 ]
机构：
[1] Zhejiang Univ, State Key Lab Diag & Treatment Infect Dis, 79 Qingchun Rd, Hangzhou 310003, Peoples R China
[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Natl Clin Res Ctr Infect Dis, 79 Qingchun Rd, Hangzhou 310003, Peoples R China
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2021年 / 33卷 / 03期
关键词：
avian influenza A virus; H2; subtype; real-time RT-PCR;
D O I：
10.1177/1040638721994810
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.
引用
收藏
页码：577 / 581
页数：5
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