In vivo time-resolved Raman imaging of a spontaneous death process of a single budding yeast cell

被引：64

作者：

Naito, Y

Toh-e, A

Hamaguchi, HO

机构：

[1] Univ Tokyo, Sch Sci, Dept Chem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Univ Tokyo, Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2005年 / 36卷 / 09期

关键词：

time- and space-resolved Raman spectroscopy; confocal Raman microspectroscopy; living cell; budding yeast; programmed cell death;

D O I：

10.1002/jrs.1397

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

In vivo confocal Raman microspectroscopy has been used to study a spontaneous death process of a single budding yeast (S. cerevisiae) cell. The observed time-resolved Raman images indicate that, concomitantly with the sudden appearance of a dancing body in a vacuole, the mitochondrial metabolic activity of the cell is markedly lowered and that the subsequent disappearance of the vacuole is followed by an inevitable cell death within a few hours. Copyright (c) 2005 John Wiley & Sons, Ltd.

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收藏

页码：837 / 839

页数：5

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