STED-SPIM: Stimulated Emission Depletion Improves Sheet Illumination Microscopy Resolution

被引：79

作者：

Friedrich, Mike ^{[1
]}

Gan, Qiang ^{[1
]}

Ermolayev, Vladimir ^{[1
]}

Harms, Gregory S. ^{[1
,2
]}

机构：

[1] Univ Wurzburg, Mol Microscopy Grp, Bioimaging Ctr, Rudolf Virchow Ctr, Wurzburg, Germany

[2] Wilkes Univ, Dept Biol & Phys, Wilkes Barre, PA 18766 USA

来源：

BIOPHYSICAL JOURNAL | 2011年 / 100卷 / 08期

关键词：

ULTRAMICROSCOPY; ZEBRAFISH;

D O I：

10.1016/j.bpj.2010.12.3748

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The integrated STED-SPIM method combines the advantages of SPIM with the resolution enhancement of STED, and thus provides a method for fast, high-resolution imaging with >100 mu m deep penetration into biological tissue.

引用

页码：L43 / L45

页数：3

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