Effect of cadmium on diaphorase activity and nitric oxide production in barley root tips

被引：60

作者：

Valentovicova, Katarina ^{[1
]}

Haluskova, L'ubica ^{[1
]}

Huttova, Jana ^{[1
]}

Mistrik, Igor ^{[1
]}

Tamas, Ladislav ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Bot, SK-84523 Bratislava, Slovakia

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2010年 / 167卷 / 01期

关键词：

Cadmium; Diaphorase; Nitric oxide; Root growth inhibition; Vascular development; SUBCELLULAR-LOCALIZATION; CELLULOSE-SYNTHESIS; NITROSATIVE STRESS; NADPH-DIAPHORASE; TOXICITY; GROWTH; PLANTS; SYNTHASE; CD; CELLS;

D O I：

10.1016/j.jplph.2009.06.018

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effect of Cd on NAD PH-diaphorase activity and nitric oxide (NO) production was investigated in barley root tips. The Cd-induced increase of NADPH-diaphorase activity occurred at the elongation zone and increased further in the differentiation zone of barley root tips. This activity was associated primarily with the microsomal membrane fraction of crude extract. In situ analysis revealed that the diaphorase activity was localized in the metaxylem and metaphloem elements and to some cells of the pericycle and parenchyma of root tips. Cd-induced NO generation was observed in pericycle, parenchymatic stelar cells and companion cells of protophloem. The results suggest that the Cd-induced generation of NO functions in Cd toxicity through the ectopic and accelerated differentiation of root tips, causing the shortening of the root elongation zone and a subsequent reduction in root growth. (C) 2009 Elsevier GmbH. All rights reserved.

引用

页码：10 / 14

页数：5

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