Dynamics of 5-Aminolevulinic Acid-Induced Accumulation of Protoporphyrin IX in Three Cell Lines of Different Origin

被引：4
|
作者：
Gorbenko, D. A. [1 ,2 ]
Belashov, A. V. [1 ]
Belyaeva, T. N. [3 ]
Kornilova, E. S. [3 ,4 ]
Litvinov, I. K. [3 ]
Semenova, I. V. [1 ]
Vasyutinskii, O. S. [1 ]
机构：
[1] Ioffe Inst, St Petersburg, Russia
[2] ITMO Univ, St Petersburg, Russia
[3] Russian Acad Sci, Inst Cytol, St Petersburg, Russia
[4] Peter the Great St Petersburg Polytech Univ, St Petersburg, Russia
来源：
TECHNICAL PHYSICS | 2021年 / 66卷 / 01期
关键词：
D O I：
10.1134/S1063784221010084
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
The dynamics of synthesis and accumulation of protoporphyrin IX induced by 5-aminolevulinic acid (5-ALA) was studied using confocal fluorescence microscopy in three established cell lines: immortalized cells originated from human tumor tissues-epithelial carcinoma (HeLa cells) and lung adenocarcinoma (A549 cells)-and immortalized fibroblast-like mouse embryo cells (3T3 line). The accumulation of protoporphyrin IX in cell cultures was estimated by analyzing the intensity of its fluorescence. The dynamics of changes in the fluorescence intensity with the cell incubation duration in the presence of 5-ALA and with the 5-ALA concentration was studied.
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页码：145 / 148
页数：4
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