Light-Regulated mRNA Condensation by a Photosensitive Surfactant Works as a Series Photoswitch of Translation Activity in the Presence of Small RNAs

被引：18

作者：

Rudiuk, Sergii ^{[2
]}

Saito, Hirohide ^{[3
]}

Hara, Tomoaki ^{[3
]}

Inoue, Tan ^{[3
]}

Yoshikawa, Kenichi ^{[2
]}

Baigl, Damien ^{[1
,4
,5
]}

机构：

[1] Ecole Normale Super, Dept Chem, F-75005 Paris, France

[2] Kyoto Univ, Dept Phys, Grad Sch Sci, Kyoto 6068502, Japan

[3] Kyoto Univ, Lab Gene Biodynam, Grad Sch Biostudies, Kyoto 6068502, Japan

[4] Univ Paris 06, F-75005 Paris, France

[5] CNRS, UMR 8640, Paris, France

来源：

BIOMACROMOLECULES | 2011年 / 12卷 / 11期

基金：

欧洲研究理事会; 日本科学技术振兴机构;

关键词：

DNA CONFORMATION; GENE-EXPRESSION; TRANSCRIPTION; PHOTOCONTROL; ACTIVATION; PROMOTER; DROPLET; BINDING;

D O I：

10.1021/bm200962s

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

AzoTAB, a photosensitive azobenzene cationic surfactant, which phototriggers translation activity through light-regulated condensation of mRNA, is added to a translation solution containing several mRNAs, which can be selectively silenced by specific small RNAs. We find that gene silencing by small RNAs remains functional regardless of AzoTAB concentration and UV illumination. In the absence of UV, the translation of all genes present in the medium is partially to fully inhibited depending on AzoTAB concentration. In contrast, the application of a short UV stimulus (365 nm for 1.5 min) results in the selective photoactivation of genes that are not silenced by small RNA. These results show that light-regulated condensation by AzoTAB works as a sequence-independent series photoswitch added to parallel sequence-specific regulation by small RNAs.

引用

页码：3945 / 3951

页数：7