A silicon-based multi-sensor chip for monitoring of fermentation processes

被引：10

作者：

Baecker, M. ^{[1
,2
]}

Pouyeshman, S. ^{[1
]}

Schnitzler, Th. ^{[1
]}

Poghossian, A. ^{[1
,2
]}

Wagner, P. ^{[3
]}

Biselli, M. ^{[1
,2
]}

Schoening, M. J. ^{[1
,2
]}

机构：

[1] Aachen Univ Appl Sci, Inst Nano & Biotechnol, D-52428 Julich, Germany

[2] Res Ctr Julich, Peter Gruenberg Inst PGI 8, D-52425 Julich, Germany

[3] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium

来源：

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE | 2011年 / 208卷 / 06期

关键词：

electrochemical sensor; electrolyte conductivity; field-effect; process monitoring; MAMMALIAN-CELL CULTURE; POTENTIOMETRIC SENSOR; OPTIMIZATION; PLATFORM; ELECTRODES; PROBES; PH;

D O I：

10.1002/pssa.201001139

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

This paper describes the design and characterization of a silicon based sensor chip for monitoring of fermentation processes. The sensor chip consists of three sensors using different transducer principles. A capacitive electrolyte-insulator-semiconductor (EIS) field-effect structure with Ta2O5 as gate material was utilized as pH sensor. An electrolyte conductivity sensor was realized by measuring the impedance between two interdigitated electrodes (IDE). A platinum thermistor was included for temperature measurements. The EIS sensor was integrated into a bioreactor and successfully used for an inline pH measurement. The layout of the IDE has been optimized with respect to a high cell constant and a wide detectable conductivity range. The integrated platinum thermistor allowed for temperature compensation of the electrolyte conductivity measurement. (C) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：1364 / 1369

页数：6

共 29 条

[1] Concept for a solid-state multi-parameter sensor system for cell-culture monitoring [J].

Baecker, M. ;

Beging, S. ;

Biselli, M. ;

Poghossian, A. ;

Wang, J. ;

Zang, W. ;

Wagner, P. ;

Schoening, M. J. .

ELECTROCHIMICA ACTA, 2009, 54 (25) :6107-6112

[2] Functional cellular assays with multiparametric silicon sensor chips [J].

Brischwein, M ;

Motrescu, ER ;

Cabala, E ;

Otto, AM ;

Grothe, H ;

Wolf, B .

LAB ON A CHIP, 2003, 3 (04) :234-240

[3] Improvement of bioprocess monitoring: development of novel concepts [J].

Clementschitsch, Franz ;

Bayer, Karl .

MICROBIAL CELL FACTORIES, 2006, 5 (1)

[4] Automated multiparametric platform for high-content and high-throughput analytical screening on living cells [J].

Geisler, T ;

Ressler, J ;

Harz, H ;

Wolf, B ;

Uhl, R .

IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING, 2006, 3 (02) :169-176

[5] Comparisons of optical pH and dissolved oxygen sensors with traditional electrochemical probes during mammalian cell culture [J].

Hanson, Michael A. ;

Ge, Xudong ;

Kostov, Yordan ;

Brorson, Kurt A. ;

Moreira, Antonio R. ;

Rao, Govind .

BIOTECHNOLOGY AND BIOENGINEERING, 2007, 97 (04) :833-841

[6] Micromachined silicon electrolytic conductivity probes with integrated temperature sensor [J].

He, DM ;

Shannon, MA ;

Miller, NR .

IEEE SENSORS JOURNAL, 2005, 5 (06) :1185-1196

[7]

Hoffmann B., 1995, 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Digest of Technical Papers (IEEE Cat. No.95TH8173), P837

[8] Optimal and consistent protein glycosylation in mammalian cell culture [J].

Hossler, Patrick ;

Khattak, Sarwat F. ;

Li, Zheng Jian .

GLYCOBIOLOGY, 2009, 19 (09) :936-949

[9] DESIGN OPTIMIZATION OF PLANAR ELECTROLYTIC CONDUCTIVITY SENSORS [J].

JACOBS, P ;

VARLAN, A ;

SANSEN, W .

MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING, 1995, 33 (06) :802-810

[10]

Kansakoski M., 2006, Process Analytical Technology needs and applications in the bioprocess industry, P1

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