Salvia miltiorrhiza polysaccharide activated macrophages and improved the disease resistance of sturgeon against Aeromonas hydrophila

被引：16

作者：

Chen, Daiyu ^{[1
]}

Yang, Lei ^{[1
]}

Yang, Fei ^{[1
]}

Pei, Qiaolin ^{[1
]}

Lu, Lu ^{[1
]}

Huang, Xiaoli ^{[1
]}

Ouyang, Ping ^{[2
]}

Geng, Yi ^{[2
]}

Li, Zhiqiong ^{[1
]}

Zhang, Xin ^{[1
]}

Wang, Jun ^{[3
]}

Chen, Defang ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu 610000, Sichuan, Peoples R China

[2] Sichuan Agr Univ, Coll Vet Med, Chengdu 610000, Sichuan, Peoples R China

[3] Neijiang Normal Univ, Neijiang 641000, Sichuan, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2022年 / 127卷

关键词：

Salvia miltiorrhiza polysaccharide; Macrophages; Disease resistance; Acipenser bareii; Hybrid sturgeon; NF-KAPPA-B; INNATE IMMUNITY; ANTIOXIDANT ACTIVITY; GROWTH-PERFORMANCE; AMUR STURGEON; NITRIC-OXIDE; HEAD KIDNEY; IN-VITRO; PHAGOCYTOSIS; IMMUNOMODULATION;

D O I：

10.1016/j.fsi.2022.06.062

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The use of plant polysaccharides in aquaculture is recognized as a healthy strategy to enhance disease resistance and reduce medication use. Salvia miltiorrhiza polysaccharide (SMP) can regulate the immune function of higher vertebrates. However, the effects of SMP on fish have not been fully investigated. In this study, the ability of SMP to activate the macrophages of Siberian sturgeon (Acipenser bareii) was analyzed in vitro. The effects of SMP on immune cell activity of hybrid sturgeon (A. baerii ? x Acipenser schrenckii ?) and resistance to Aeromonas hydrophila were further detected in vivo. The in vitro results showed that SMP up-regulated phagocytosis, respiratory burst, inducible nitric oxide synthase activity, nitric oxide (NO) concentration, and cytokine mRNA expression of macrophages. The in vivo results showed that dietary supplementation with SMP enhanced the respiratory burst of macrophages and proliferative activity of lymphocytes. Dietary supplementation with SMP increased serum concentrations of lysozyme and NO, and improved the survival rate of hybrid sturgeon challenged with A. hydrophila. Collectively, these results suggest that SMP can improve the immune function and disease resistance of sturgeon. This study provides a theoretical basis for the application of SMP for healthy farming of sturgeon.

引用

页码：594 / 603

页数：10

共 66 条

[1] Macrophages of the splenic marginal zone are essential for trapping of blood-borne particulate antigen but dispensable for induction of specific T cell responses [J].

Aichele, P ;

Zinke, J ;

Grode, L ;

Schwendener, RA ;

Kaufmann, SHE ;

Seiler, P .

JOURNAL OF IMMUNOLOGY, 2003, 171 (03) :1148-1155

[2] Interleukin-6 protects liver against warm ischemia/reperfusion injury and promotes hepatocyte proliferation in the rodent [J].

Camargo, CA ;

Madden, JF ;

Gao, WS ;

Selvan, RS ;

Clavien, PA .

HEPATOLOGY, 1997, 26 (06) :1513-1520

[3] The growth performance and nonspecific immunity of juvenile grass carp (Ctenopharyngodon idella) affected by dietary Porphyra yezoensis polysaccharide supplementation [J].

Chen, Li ;

Zhang, Yipeng .

FISH & SHELLFISH IMMUNOLOGY, 2019, 87 :615-619

[4] Salvia miltiorrhiza polysaccharide activates T Lymphocytes of cancer patients through activation of TLRs mediated -MAPK and -NF-κB signaling pathways [J].

Chen, Yanan ;

Li, Haifeng ;

Li, Meifeng ;

Niu, Shubin ;

Wang, Jiaxin ;

Shao, Hongwei ;

Li, Ting ;

Wang, Hui .

JOURNAL OF ETHNOPHARMACOLOGY, 2017, 200 :165-173

[5] Characterization of a polysaccharide HP-02 from Honeysuckle flowers and its immunoregulatory and anti-Aeromonas hydrophila effects in updates Cyprinus carpio L. [J].

Feng, Junchang ;

Chang, Xulu ;

Zhang, Yuru ;

Lu, Ronghua ;

Meng, Xiaolin ;

Song, Dongying ;

Yan, Xiao ;

Zhang, Jianxin ;

Nie, Guoxing .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 140 :477-483

[6] Phenotypic and functional plasticity of cells of innate immunity: macrophages, mast cells and neutrophils [J].

Galli, Stephen J. ;

Borregaard, Niels ;

Wynn, Thomas A. .

NATURE IMMUNOLOGY, 2011, 12 (11) :1035-1044

[7] Phagocytosis: An Immunobiologic Process [J].

Gordon, Siamon .

IMMUNITY, 2016, 44 (03) :463-475

[8] Analysis of Acanthopanax giraldii Harms Polysaccharide II Composition and Its Immune-Protective Role in a Cyclophosphamide-Induced Immunosuppressive Mice Model [J].

Guo, Xiaoxiao ;

Hao, Huihui ;

Meng, Aixia ;

Xu, Cai ;

Ma, Yuan ;

Sun, Fengxiang ;

Chen, Yong .

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2021, 2021

[9] Activation of murine macrophages by G1-4A, a polysaccharide from Tinospora cordifolia, in TLR4/MyD88 dependent manner [J].

Gupta, Pramod Kumar ;

Rajan, M. G. R. ;

Kulkarni, Savita .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2017, 50 :168-177

[10] Inhibition of anti-inflammatory cytokines, IL-10 and TGF-?, in Leishmania major infected macrophage by miRNAs: A new therapeutic modality against leishmaniasis [J].

Hamidi, Faezeh ;

Mohammadi-Yeganeh, Samira ;

Hoseini, Mostafa Haji Molla ;

Tabaei, Seyyed Javad Seyyed ;

Taghipour, Niloofar ;

Lasjerdi, Zohreh ;

Gholamrezaei, Mostafa ;

Haghighi, Ali .

MICROBIAL PATHOGENESIS, 2021, 153

← 1 2 3 4 5 6 7 →