New Structural Proteins of Halobacterium salinarum Gas Vesicle Revealed by Comparative Proteomics Analysis

被引：25

作者：

Chu, Lichieh Julie ^{[1
]}

Chen, Mengchieh Claire ^{[1
]}

Setter, Jocelyn ^{[4
]}

Tsai, Yihsuan Shannon ^{[2
,4
]}

Yang, Hanyin ^{[2
]}

Fang, Xuefeng ^{[4
,5
]}

Ting, Ying Sonia ^{[2
,4
]}

Shaffer, Scott A. ^{[4
]}

Taylor, Gregory K. ^{[4
]}

von Haller, Priska D. ^{[6
]}

Goodlett, David R. ^{[4
]}

Ng, Wailap Victor ^{[1
,2
,3
,7
]}

机构：

[1] Natl Yang Ming Univ, Inst Biotechnol Med, Taipei 112, Taiwan

[2] Natl Yang Ming Univ, Inst Biomed Informat, Taipei 112, Taiwan

[3] Natl Yang Ming Univ, Dept Biotechnol & Lab Sci Med, Taipei 112, Taiwan

[4] Univ Washington, Dept Med Chem, Seattle, WA 98195 USA

[5] Zhejiang Univ, Zhejiang California Nanosyst Inst, Syst Biol Div, Hangzhou 310029, Zhejiang, Peoples R China

[6] Univ Washington, UW Prote Resource, Seattle, WA 98109 USA

[7] Taipei City Hosp, Clin Biotechnol Res Ctr, Taipei, Taiwan

来源：

JOURNAL OF PROTEOME RESEARCH | 2011年 / 10卷 / 03期

关键词：

gas vesicle; structural protein; archaea; Halobacterium salinarum; proteomics; TRANSCRIPTIONAL ACTIVATOR GVPE; GENE-CLUSTER; MASS-SPECTROMETRY; PURPLE MEMBRANE; P-LOOP; HALOBIUM; REGION; IDENTIFICATION; ARCHAEBACTERIUM; EXPRESSION;

D O I：

10.1021/pr1009383

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Halobacterium salinarum gas vesicle (GV) is an extremely stable intracellular organelle with air trapped inside a proteinaceous membrane. Reported here is a comparative proteomics analysis of GV and GV depleted lysate (GVD) to reveal the membrane structural proteins. Ten proteins encoded by gvp-1 (gvpMLKJIHGFED-1 and gvpACNO-1) and five proteins encoded by gvp-2 (gvpMLKJIHGFED-2 and gvpACNO-2) gene clusters for the biogenesis of spindle- and cylindrical-, respectively, shaped GV were identified by LC-MS/MS. The peptides of GypA1, I1, J1, A2, and J2 were exclusively identified in purified GV, GypD1, H1, L1, and F2 only in GVD, and GypC1, N1, O1, F1, H2, and O2 in both samples. The identification of GypA1, C1, F1, J1, and A2 in GV is in agreement with their previously known structural function. In addition, the detection of GypI1, N1, O1, H2, J2, and O2 in GV suggested they are new structural proteins. Among these, the structural role of GypI1 and N1 in CV was further validated by immuno-detection of protein A-tagged GvpI1 and N1 fusion proteins in purified GV. Thus, LC-MS/MS could reveal at least a half dozen gas vesicle structural proteins in the predominant spindle-shaped GV that may be helpful for studying its biogenesis.

引用

页码：1170 / 1178

页数：9

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