Evaluation of the genetic fidelity of in vitro-propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers

被引：76

作者：

Bhatia, R. ^{[1
]}

Singh, K. P. ^{[2
]}

Sharma, T. R. ^{[3
]}

Jhang, Tripta ^{[3
]}

机构：

[1] Indian Agr Res Inst, Reg Stn, Kulluvalley 175129, Himachal Prades, India

[2] Indian Agr Res Inst, Div Floriculture & Landscaping, New Delhi 110012, India

[3] Indian Agr Res Inst, Natl Res Ctr Plant Biotechnol, New Delhi 110012, India

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2011年 / 104卷 / 01期

关键词：

Gerbera; Micropropagation; Genetic fidelity; DNA markers; PLANT-REGENERATION; RAPD; ISSR; STABILITY; CALLUS;

D O I：

10.1007/s11240-010-9806-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genetic fidelity of in vitro-raised gerbera clones was assessed by using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Out of 35 RAPD and 32 ISSR primers screened, only 12 RAPD and 10 ISSR primers produced clear, reproducible and scorable bands. The 12 RAPD primers produced 54 distinct and scorable bands, with an average of 4.5 bands per primer. The number of scorable bands for ISSR primers varied from 3 (ISSR-14) to 9 (ISSR-07), with an average of 5.5 bands per primer. The number of bands generated per primer was greater in ISSR than RAPD. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant. A similarity matrix based on Jaccard's coefficient revealed that the pair-wise value between the mother and the in vitro-raised plantlets was 1, indicating 100% similarity. This confirmed the true-to-type nature of the in vitro-raised clones.

引用

页码：131 / 135

页数：5

共 13 条

[1]

[Anonymous], 1988, NTSYS-PC: Numerical taxonomy and multivariate analysis system

[2]

Bhatia R, 2008, INDIAN J HORTIC, V65, P312

[3] RAPD, ISSR and RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type) and C-assamica ssp assamica (Assam-India type) [J].

Devarumath, RM ;

Nandy, S ;

Rani, V ;

Marimuthu, S ;

Muraleedharan, N ;

Raina, SN .

PLANT CELL REPORTS, 2002, 21 (02) :166-173

[4] Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers [J].

Goto, S ;

Thakur, RC ;

Ishii, K .

PLANT CELL REPORTS, 1998, 18 (3-4) :193-197

[5] Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay [J].

Joshi, P. ;

Dhawan, V. .

BIOLOGIA PLANTARUM, 2007, 51 (01) :22-26

[6] In vitro propagation of Gerbera -: A review [J].

Kanwar, J. K. ;

Kumar, S. .

HORTICULTURAL SCIENCE, 2008, 35 (01) :35-44

[7] Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers [J].

Lakshmanan, Venkatachalam ;

Venkataramareddy, Sreedhar Reddampalli ;

Neelwarne, Bhagyalakshmi .

ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2007, 10 (01) :106-113

[8] Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers [J].

Martins, M ;

Sarmento, D ;

Oliveira, MM .

PLANT CELL REPORTS, 2004, 23 (07) :492-496

[9] RAPID ISOLATION OF HIGH MOLECULAR-WEIGHT PLANT DNA [J].

MURRAY, MG ;

THOMPSON, WF .

NUCLEIC ACIDS RESEARCH, 1980, 8 (19) :4321-4325

[10] PLANT-REGENERATION FROM INVITRO LEAF CULTURE OF SEVERAL GERBERA SPECIES [J].

REYNOIRD, JP ;

CHRIQUI, D ;

NOIN, M ;

BROWN, S ;

MARIE, D .

PLANT CELL TISSUE AND ORGAN CULTURE, 1993, 33 (02) :203-210

← 1 2 →