miR-183 induces cell proliferation, migration, and invasion by regulating PDCD4 expression in the SW1990 pancreatic cancer cell line

被引：35

作者：

Lu, Ying-Ying ^{[1
]}

Zheng, Jun-Yuan ^{[1
]}

Liu, Jie ^{[1
]}

Huang, Chun-Lan ^{[1
]}

Zhang, Wei ^{[2
]}

Zeng, Yue ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Dept Gastroenterol, Shanghai Peoples Hosp 1, Shanghai 200080, Peoples R China

[2] Shanghai Fudan Univ, Sch Med, Dept Gastroenterol, Huadong Hosp, Shanghai 200040, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2015年 / 70卷

基金：

美国国家科学基金会;

关键词：

Pancreatic cancer; MiR-183; PDCD4; HEPATOCELLULAR-CARCINOMA CELLS; PROSTATE-CANCER; COLORECTAL-CANCER; DOWN-REGULATION; MICRORNAS; IDENTIFICATION; TUMORIGENESIS; SUPPRESSES; APOPTOSIS; MARKER;

D O I：

10.1016/j.biopha.2015.01.016

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The aim of this study was to investigate the function of miR-183 in the SW1990 cancer cell line, and the mechanisms regulating these processes. miRNAs are known to play important roles in cancer cell development. However, the pattern and biological role of miR-183 in pancreatic cancer remain largely unknown. Here, we have reported the reduction in pancreatic cancer cell growth in vitro by miR-183 intervention, by inducing apoptosis and decreasing the Bcl-2 expression. Moreover, miR-183 was observed to enhance pancreatic cancer cell migration and invasion, whereas inhibition of miR-183 caused an opposite effect. miR-183 inhibition was shown to increase E-cadherin expression and decrease N-cadherin expression. These regulatory actions play an important role in the cancer epithelial-mesenchymal transition (EMT). Mechanistically, we demonstrated that the overexpression of miR-183 decreased the expression of PDCD4 (programmed cell death 4) mRNA and protein, and vice versa. This helped to identify PDCD4 as the target genes in pancreatic cancer. In conclusion, our analyses indicated miR-183 to be an important contributor to cell migration. This could also be used as a potential therapeutic target for pancreatic cancer treatment. (C) 2015 Elsevier Masson SAS. All rights reserved.

引用

页码：151 / 157

页数：7

共 28 条

[1] Pdcd4, a colon cancer prognostic that is regulated by a microRNA [J].

Allgayer, Heike .

CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, 2010, 73 (03) :185-191

[2] Identification by Real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues [J].

Bandres, E. ;

Cubedo, E. ;

Agirre, X. ;

Malumbres, R. ;

Zarate, R. ;

Ramirez, N. ;

Abajo, A. ;

Navarro, A. ;

Moreno, I. ;

Monzo, M. ;

Garcia-Foncillas, J. .

MOLECULAR CANCER, 2006, 5 (1)

[3] Oncomirs - microRNAs with a role in cancer [J].

Esquela-Kerscher, A ;

Slack, FJ .

NATURE REVIEWS CANCER, 2006, 6 (04) :259-269

[4] Blockade of hedgehog signaling inhibits pancreatic cancer invasion and metastases: A new paradigm for combination therapy in solid cancers [J].

Feldmann, Georg ;

Dhara, Surajit ;

Fendrich, Volker ;

Bedja, Djahida ;

Beaty, Robert ;

Mullendore, Michael ;

Karikari, Collins ;

Alvarez, Hector ;

Iacobuzio-Donahue, Christine ;

Jimeno, Antonio ;

Gabrielson, Kathleen L. ;

Matsui, William ;

Maitra, Anirban .

CANCER RESEARCH, 2007, 67 (05) :2187-2196

[5]

Gu W, 2014, INT J CLIN EXP MED, V7, P2519

[6] Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis [J].

Jansen, AP ;

Camalier, CE ;

Colburn, NH .

CANCER RESEARCH, 2005, 65 (14) :6034-6041

[7] The tumour suppressor Pdcd4: recent advances in the elucidation of function and regulation [J].

Lankat-Buttgereit, Brigitte ;

Goeke, Ruediger .

BIOLOGY OF THE CELL, 2009, 101 (06) :309-317

[8] Identification of differentially expressed microRNAs in human male breast cancer [J].

Lehmann, Ulrich ;

Streichert, Thomas ;

Otto, Benjamin ;

Albat, Cord ;

Hasemeier, Britta ;

Christgen, Henriette ;

Schipper, Elisa ;

Hille, Ursula ;

Kreipe, Hans H. ;

Laenger, Florian .

BMC CANCER, 2010, 10

[9] Targeting of Integrin β1 and Kinesin 2α by MicroRNA 183 [J].

Li, Guorong ;

Luna, Coralia ;

Qiu, Jianming ;

Epstein, David L. ;

Gonzalez, Pedro .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (08) :5461-5471

[10] miR-183 inhibits TGF-β1-induced apoptosis by downregulation of PDCD4 expression in human hepatocellular carcinoma cells [J].

Li, Jipeng ;

Fu, Hanjiang ;

Xu, Chengwang ;

Tie, Yi ;

Xing, Ruiyun ;

Zhu, Jie ;

Qin, Yide ;

Sun, Zhixian ;

Zheng, Xiaofei .

BMC CANCER, 2010, 10

← 1 2 3 →