GLI-1 facilitates the EMT induced by TGF-β1 in gastric cancer

被引：1
作者：
Liang, M. [1 ]
Liu, X-C [1 ]
Liu, T. [2 ]
Li, W-J [3 ]
Xiang, J-G [1 ]
Xiao, D. [1 ]
Zhang, Y-L [1 ]
Zheng, M-H [1 ]
Zhai, C. [1 ]
Chen, L. [1 ]
Bai, Y-H [4 ]
机构：
[1] Xi An Jiao Tong Univ, Hosp 3201, Coll Med, Dept Oncol Surg, Hanzhong City, Peoples R China
[2] Xi An Jiao Tong Univ, Hosp 3201, Coll Med, Dept Ophthalmol, Hanzhong City, Peoples R China
[3] Xi An Jiao Tong Univ, Hosp 3201, Coll Med, Dept Pathol, Hanzhong City, Peoples R China
[4] Xi An Jiao Tong Univ, Hosp 3201, Coll Med, Dept Ultrasonog, Hanzhong City, Peoples R China
来源：
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES | 2018年 / 22卷 / 20期
关键词：
Gastric cancer; Transforming growth factor-beta1; Glioma associated oncogene homolog 1; Epithelial mesenchymal transformation; Invasion; MESENCHYMAL TRANSITION; HEMORRHAGIC-SHOCK; METAANALYSIS; CHINA;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
OBJECTIVE: To explore how GLI-1 affects the EMT induced by TGF-beta 1 in gastric cancer. MATERIALS AND METHODS: Following 24 hours of culture of SGC-7901 cells in presence of TGF-beta 1, we observed the changes in morphology as well as mRNA and protein expressions of GLI-1, E-cadherin and Vimentin by RT-PCR and Western blot. Transwell assay was conducted to evaluate the changes in invasion ability of SGC-7901 cells. Then. SGC-7901 cells were co-treated with TGF-beta 1 and GANT 61, and changes of the above indexes were also detected using the corresponding methods. RESULTS: In presence of TGF-beta 1, EMT was initiated in SGC-7901 cells EMT with increased cell invasion ability, and the mRNA and protein expressions of E-cadherin were downregulated, while those of the GLI-1 and Vimentin were upregulated. Conversely, the co-treatment of TGF-beta 1 and GANT 61 suppressed the increased cell invasion ability induced only by TGF-beta 1, and the changes in mRNA and protein expressions of these factors were abolished. CONCLUSIONS: We found that GLI-1 facilitates the EMT induced by TGF-beta 1 in SGC-7901 cells, which may serve as a potential target in developing the clinical treatment of gastric cancer.
引用
收藏
页码：6809 / 6815
页数：7
相关论文
共 50 条
  • [41] Association between endogenous gene expression and growth regulation induced by TGF-β1 in human gastric cancer cells
    Li, Xue
    Zhang, Yun-Yan
    Wang, Qi
    Fu, Song-Bin
    [J]. WORLD JOURNAL OF GASTROENTEROLOGY, 2005, 11 (01) : 61 - 68
  • [42] Involvement of eIF2α in halofuginone-driven inhibition of TGF-β1-induced EMT
    Duan, Mingyuan
    Wei, Xiaobing
    Cheng, Zhe
    Liu, Dunjiang
    Fotina, Hanna
    Xia, Xiaojing
    Hu, Jianhe
    [J]. JOURNAL OF BIOSCIENCES, 2020, 45 (01)
  • [43] Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF-β signalling
    Yan, Wenji
    Wu, Kongming
    Herman, James G.
    Brock, Malcolm V.
    Zhou, Yusen
    Lu, Youyong
    Zhang, Zhiqian
    Yang, Yunsheng
    Guo, Mingzhou
    [J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2014, 18 (12) : 2499 - 2511
  • [44] PEITC inhibits the invasion and migration of colorectal cancer cells by blocking TGF-β-induced EMT
    Xiao, Jian
    Zhou, Ningning
    Li, Yin
    Xiao, Yunyun
    Chen, Wei
    Ye, Junwen
    Ma, Tenghui
    Zhang, Yan
    [J]. BIOMEDICINE & PHARMACOTHERAPY, 2020, 130
  • [45] Sialyltransferase ST3GAL1 promotes cell migration, invasion, and TGF-β1-induced EMT and confers paclitaxel resistance in ovarian cancer
    Wu, Xin
    Zhao, Junda
    Ruan, Yuanyuan
    Sun, Li
    Xu, Congjian
    Jiang, Hua
    [J]. CELL DEATH & DISEASE, 2018, 9
  • [46] Troglitazone Attenuates TGF-β1-Induced EMT in Alveolar Epithelial Cells via a PPARγ-Independent Mechanism
    Zhou, Beiyun
    Buckley, Stephen T.
    Patel, Vipul
    Liu, Yixin
    Luo, Jiao
    Krishnaveni, Manda Sai
    Ivan, Mihaela
    DeMaio, Lucas
    Kim, Kwang-Jin
    Ehrhardt, Carsten
    Crandall, Edward D.
    Borok, Zea
    [J]. PLOS ONE, 2012, 7 (06):
  • [47] The crucial role of SRPK1 in IGF-1-induced EMT of human gastric cancer
    Wang, Hong
    Wang, Chunlei
    Tian, Wenling
    Yao, Yanfen
    [J]. ONCOTARGET, 2017, 8 (42) : 72157 - 72166
  • [48] TGF-β1 gene silencing can enhances the sensitivity of breast cancer to cisplatin partially by restraining the occurrence of EMT
    Chen, Chao
    Feng, Zhen-Yu
    Jiang, Guo-Qin
    Gu, Yan-Lin
    Jin, Ke
    Chu, Dong-Ming
    [J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2017, 10 (11): : 10751 - 10758
  • [49] Hypoxia-induced HIF-1α and ZEB1 are critical for the malignant transformation of ameloblastoma via TGF-β-dependent EMT
    Yoshimoto, Shohei
    Tanaka, Fumie
    Morita, Hiromitsu
    Hiraki, Akimitsu
    Hashimoto, Shuichi
    [J]. CANCER MEDICINE, 2019, 8 (18): : 7822 - 7832
  • [50] TGF-β1-Induced Upregulation of MALAT1 Promotes Kazakh's Esophageal Squamous Cell Carcinoma Invasion by EMT
    Liu, Qing
    Zheng, Shutao
    Chen, Yumei
    Liu, Tao
    Han, Xiujuan
    Zhang, Xiao
    Shen, Tongxue
    Lu, Xiaomei
    [J]. JOURNAL OF CANCER, 2020, 11 (23): : 6892 - 6901
12345