A Selective Imidazoline-2-thione-Bearing Two-Photon Fluorescent Probe for Hypochlorous Acid in Mitochondria

被引：168

作者：

Xu, Qingling ^{[1
]}

Heo, Cheol Ho ^{[2
]}

Kim, Jin A. ^{[1
]}

Lee, Hye Sue ^{[2
]}

Hu, Ying ^{[1
]}

Kim, Dayoung ^{[1
]}

Swamy, Kunemadihalli Mathada Kotraiah ^{[1
,3
]}

Kim, Gyoungmi ^{[1
]}

Nam, Sang-Jip ^{[1
]}

Kim, Hwan Myung ^{[2
]}

Yoon, Juyoung ^{[1
]}

机构：

[1] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea

[2] Ajou Univ, Dept Energy Syst Res, Suwon 443749, Gyeonggi Do, South Korea

[3] VL Coll Pharm, Dept Pharmaceut Chem, Raichur 584103, Karnataka, India

来源：

ANALYTICAL CHEMISTRY | 2016年 / 88卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

INDUCED HOCL PRODUCTION; LIVE CELLS; REACTIVE OXYGEN; LIVING CELLS; CANCER-CELLS; IN-VIVO; PH; TISSUES; MYELOPEROXIDASE; CHEMISTRY;

D O I：

10.1021/acs.analchem.6b01738

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hypochlorite (OCl-) plays a key role in the immune system and is involved in various diseases. Accordingly, direct detection of endogenous OCl- at the subcellular level is important for understanding inflammation and cellular apoptosis. In the current study, a two-photon fluorescent off/on probe (PNIS) bearing imidazoline-2-thione as an OCl- recognition unit and triphenylphosphine (TPP) as a mitochondrial-targeting group was synthesized and examined for its ability to image mitochondrial OCl- in situ. This probe, based on the specific reaction between imidazoline-2-thione and OCl-, displayed a selective fluorescent off/on response to OCl- with the various reactive oxygen species in a physiological medium. PNIS was successfully applied to image of endogenously produced mitochondrial OCl- in live RAW 264.7 cells-via two-photon microscopy.

引用

页码：6615 / 6620

页数：6

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