Isolation of alkaloids of Erythrina from tissue culture

被引:0
作者
García-Mateos, R
Soto-Hernández, M
Martínez-Vázquez, M
Villegas-Monter, A
机构
[1] Colegio Postgrad, Programa Bot, Montecillio 56230, Mexico
[2] Univ Autonoma Chapingo, Chapingo 56230, Mexico
[3] Univ Nacl Autonoma Mexico, Inst Quim, Coyoacan 04510, DF, Mexico
[4] Colegio Postgrad, Programa Fruticultura, Montecillio 56230, Mexico
关键词
callus; Erythrina americana; Erythrina coralloides; gas chromatography mass spectrometry; Leguminosae;
D O I
10.1002/(SICI)1099-1565(199901/02)10:1<12::AID-PCA425>3.0.CO;2-A
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Seeds of two species of Erythrina (Leguminosae) were examined for the presence of biologically active alkaloids. Undifferentiated callus of Erythrina coralloides and E. americana produced the tetracyclic alkaloids typical for each species, but an unexpected profile of oxygenated alkaloids was observed. The tissue was analysed after isolation and purification, and identification of the alkaloids was by gas chromatography-mass spectrometry (GC-MS). A higher amount of alkaloid was observed in seeds compared with callus off. americana, A considerable difference is observed when the alkaloid content from callus is compared with that of the intact plant. (C) 1999 John Wiley & Sons, Ltd.
引用
收藏
页码:12 / 16
页数:5
相关论文
共 19 条
[1]   ALKALOIDS FROM THE FLOWERS OF ERYTHRINA-AMERICANA [J].
AGUILAR, MI ;
GIRAL, F ;
ESPEJO, O .
PHYTOCHEMISTRY, 1981, 20 (08) :2061-2062
[2]  
BLANDRIN MF, 1988, BIOTECHNOLOGY AGR FO, V4, P1
[3]  
Craig L. E., 1955, ALKALOIDS, V5, P265
[4]  
FOLKERS K, 1938, J AM PHARM ASSOC, V28, P1019
[5]  
GAMES DE, 1974, LLOYDIA, V37, P581
[6]  
Garcia E., 1973, MODIFICACIONES SISTE
[7]   Variation of total nitrogen, non-protein nitrogen content, and types of alkaloids at different stages of development in Erythrina americana seeds [J].
GarciaMateos, R ;
Lucas, B ;
Zendejas, M ;
SotoHernandez, M ;
Martinez, M ;
Sotelo, A .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (10) :2987-2991
[8]  
GARCIAMATEOS R, 1996, THESIS COLEGIO POSTG
[9]  
HARGREAVES RT, 1974, LLOYDIA, V37, P569
[10]  
LEHMAN A, 1937, J PHARM, V60, P60