Human Feeder Cells Can Support the Undifferentiated Growth of Human and Mouse Embryonic Stem Cells Using Their Own Basic Fibroblast Growth Factors

被引：24

作者：

Park, Yong ^{[1
,2
]}

Kim, Ji Hea ^{[2
,3
]}

Lee, Seung Jin ^{[2
,3
]}

Choi, In Young ^{[2
]}

Park, Seh Jong ^{[1
,2
]}

Lee, Se Ryeon ^{[1
,2
]}

Sung, Hwa Jung ^{[1
,2
]}

Yoo, Young Do ^{[2
,3
]}

Geum, Dong Ho ^{[3
]}

Choi, Chul Won ^{[1
,2
]}

Kim, Sun Haeng ^{[2
,4
]}

Kim, Byung Soo ^{[1
,2
]}

机构：

[1] Korea Univ, Dept Internal Med, Med Ctr, Seoul 136705, South Korea

[2] Korea Univ, Inst Stem Cell Res, Seoul 136705, South Korea

[3] Korea Univ, Grad Sch Med, Seoul 136705, South Korea

[4] Korea Univ, Dept Obstet & Gynecol, Med Ctr, Seoul 136705, South Korea

来源：

STEM CELLS AND DEVELOPMENT | 2011年 / 20卷 / 11期

关键词：

HUMAN PLACENTA; CULTURE; LINES; PROPAGATION; DERIVATION; FGF; LIF;

D O I：

10.1089/scd.2010.0496

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

In the culture system using human feeder cells, the mechanism through which these cells support undifferentiated growth of embryonic stem cells (ESCs) has not been well investigated. Here, we explored the mechanisms of 3 kinds of human feeder cells, including human placental cells from the chorionic plate, human bone marrow stromal cells, and human foreskin fibroblasts. First, we determined that undifferentiated growth of 2 kinds each of human (H1 and HSF6) and mouse (D3 and CE3) ESCs was possible in all human feeder cell types tested (human placental cells, human bone marrow stromal cells, and human foreskin fibroblasts), without the need for exogenous cytokine supplementation including basic fibroblast growth factor (bFGF) and leukemia inhibitory factor. We then prepared their corresponding endogenous bFGF-knockout feeders using siRNA and tried to maintain human and mouse ESCs in their undifferentiated state; however, neither human nor mouse ESCs could be maintained in bFGF-knockout human feeder cells. The expressions of stemness markers such as Oct-4 and Nanog were significantly decreased in the bFGF-knockout group compared with those in the controls, and differentiation had already occurred, despite the undifferentiated morphologic appearance of the ESCs. In conclusion, human feeder cells are able to support the undifferentiated growth of human and mouse ESCs via bFGF synthesis. Further, a bFGF-dependent pathway might be crucial for maintaining the undifferentiated characteristics of mouse and human ESCs.

引用

页码：1901 / 1910

页数：10

共 22 条

[1] Properties of four human embryonic stem cell lines maintained in a feeder-free culture system [J].