Myelin Quantification in White Matter Pathology of Progressive Multiple Sclerosis Post-Mortem Brain Samples: A New Approach for Quantifying Remyelination

被引:7
|
作者
Huitema, Marije J. D. [1 ]
Strijbis, Eva M. M. [2 ]
Luchicchi, Antonio [1 ]
Bol, John G. J. M. [1 ]
Plemel, Jason R. [3 ,4 ]
Geurts, Jeroen J. G. [1 ]
Schenk, Geert J. [1 ]
机构
[1] Vrije Univ Amsterdam, Med Ctr, Amsterdam UMC, Dept Anat & Neurosci,Amsterdam Neurosci,MS Ctr Am, De Boelelaan 1108, NL-1081 HV Amsterdam, Netherlands
[2] Vrije Univ Amsterdam, Med Ctr, Amsterdam UMC, Dept Neurol,MS Ctr Amsterdam, NL-1081 HZ Amsterdam, Netherlands
[3] Univ Alberta, Neurosci & Mental Hlth Inst, Div Neurol, Dept Med, Edmonton, AB T6G 2S2, Canada
[4] Univ Alberta, Dept Med Microbiol & Immunol, Edmonton, AB T6G 2S2, Canada
基金
加拿大健康研究院;
关键词
multiple sclerosis; remyelination; demyelination; myelin quantification; repair; MAGNETIZATION-TRANSFER RATIO; CORTICAL REMYELINATION; CNS REMYELINATION; AGREEMENT; SYSTEM;
D O I
10.3390/ijms222312634
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Multiple sclerosis (MS) is a demyelinating and neurodegenerative disease of the central nervous system (CNS). Repair through remyelination can be extensive, but quantification of remyelination remains challenging. To date, no method for standardized digital quantification of remyelination of MS lesions exists. This methodological study aims to present and validate a novel standardized method for myelin quantification in progressive MS brains to study myelin content more precisely. Fifty-five MS lesions in 32 tissue blocks from 14 progressive MS cases and five tissue blocks from 5 non-neurological controls were sampled. MS lesions were selected by macroscopic investigation of WM by standard histopathological methods. Tissue sections were stained for myelin with luxol fast blue (LFB) and histological assessment of de- or remyelination was performed by light microscopy. The myelin quantity was estimated with a novel myelin quantification method (MQM) in ImageJ. Three independent raters applied the MQM and the inter-rater reliability was calculated. We extended the method to diffusely appearing white matter (DAWM) and encephalitis to test potential wider applicability of the method. Inter-rater agreement was excellent (ICC = 0.96) and there was a high reliability with a lower- and upper limit of agreement up to -5.93% to 18.43% variation in myelin quantity. This study builds on the established concepts of histopathological semi-quantitative assessment of myelin and adds a novel, reliable and accurate quantitative measurement tool for the assessment of myelination in human post-mortem samples.
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页数:16
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