Brain-wide TVA compensation allows rabies virus to retrograde target cell-type-specific projection neurons

被引：11

作者：

Han, Zengpeng ^{[1
,2
,3
,4
]}

Luo, Nengsong ^{[1
,3
,4
,5
]}

Kou, Jiaxin ^{[1
,6
]}

Li, Lei ^{[1
]}

Xu, Zihong ^{[7
]}

Wei, Siyuan ^{[8
]}

Wu, Yang ^{[1
]}

Wang, Jie ^{[1
,2
]}

Ye, Chaohui ^{[1
,2
]}

Lin, Kunzhang ^{[3
,4
]}

Xu, Fuqiang ^{[1
,2
,3
,4
,5
,9
,10
]}

机构：

[1] Chinese Acad Sci, Wuhan Natl Lab Optoelect,Key Lab Magnet Resonance, Innovat Acad Precis Measurement Sci & Technol,Sta, Wuhan Inst Phys & Math,Natl Ctr Magnet Resonance, Wuhan 430071, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Shenzhen Key Lab Viral Vectors Biomed, Brain Cognit & Brain Dis Inst BCBDI, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China

[4] Guangdong Prov Med Prod Adm, Key Lab Qual Control Technol Virus Based Therapeu, NMPA Key Lab Res & Evaluat Viral Vector Technol C, Shenzhen Hong Kong Inst Brain Sci,Shenzhen Fundam, Shenzhen 518055, Peoples R China

[5] Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Wuhan 430074, Peoples R China

[6] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Basic Med, Educ Minist,Key Lab Neurol Disorder,Dept Pathophy, Wuhan, Peoples R China

[7] Wuhan Univ, Coll Life Sci, Wuhan, Peoples R China

[8] Wuhan Univ, HongYi Honor Coll, Wuhan, Peoples R China

[9] Shenzhen Hong Kong Inst Brain Sci, Shenzhen Fundamental Res Inst, Shenzhen 518055, Peoples R China

[10] Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China

来源：

MOLECULAR BRAIN | 2022年 / 15卷 / 01期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Retrograde tracing; Rabies virus; AAV-PHP; eB; Cell-type-specific neurons; Projection neurons; DOPAMINE NEURONS; GENE-EXPRESSION; MOUSE MODEL; IN-VIVO; CIRCUITS; DELIVERY; COMPLEMENTATION; TRANSDUCTION; EFFICIENCY; VARIANTS;

D O I：

10.1186/s13041-022-00898-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Retrograde tracers based on viral vectors are powerful tools for the imaging and manipulation of upstream neural networks projecting to a specific brain region, and they play important roles in structural and functional studies of neural circuits. However, currently reported retrograde viral tracers have many limitations, such as brain area selectivity or the inability to retrograde label genetically defined brain-wide projection neurons. To overcome these limitations, a new retrograde tracing method, AAV-PHP.eB assisted retrograde tracing systems (PARTS) based on rabies virus, was established through brain-wide TVA-dependent targeting using an AAV-PHP.eB that efficiently crosses the blood-brain barrier in C57BL/6 J mice, and complementation of EnvA-pseudotyped defective rabies virus that specifically recognizes the TVA receptor. Furthermore, combined with Cre transgenic mice, cell-type-specific PARTS (cPARTS) was developed, which can retrograde label genetically defined brain-wide projection neurons. Our research provides new tools and technical support for the analysis of neural circuits.

引用

页数：13

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