QUANTIFICATION OF TRYPTOPHAN IN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

被引：11

作者：

Pinhati, Renata Romanholi ^{[1
]}

Polonini, Hudson Caetano ^{[1
]}

Fernandes Brandao, Marcos Antonio ^{[1
]}

Barbosa Raposo, Nadia Rezende ^{[1
]}

Corchs, Felipe ^{[2
]}

Gattaz, Wagner Farid ^{[2
]}

机构：

[1] Univ Fed Juiz de Fora, Fac Farm, BR-36036330 Juiz De Fora, MG, Brazil

[2] Univ Sao Paulo, Fac Med, Dept & Inst Psiquiatria, BR-01246903 Sao Paulo, Brazil

来源：

QUIMICA NOVA | 2012年 / 35卷 / 03期

关键词：

tryptophan; serotonin; HPLC; KYNURENINE; SERUM; DISEASE; SAMPLES; HPLC;

D O I：

10.1590/S0100-40422012000300032

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.

引用

页码：623 / 626

页数：4

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