Live non-invasive Shigella dysenteriae 1 strain induces homologous protective immunity in a guinea pig colitis model

被引：6

作者：

Barman, Soumik ^{[1
]}

Kumar, Ranajit ^{[1
]}

Chowdhury, Goutam ^{[1
]}

Saha, Dhira Rani ^{[1
]}

Wajima, Takeaki ^{[2
]}

Hamabata, Takashi ^{[2
]}

Ramamurthy, Thandavarayan ^{[1
]}

Nair, Gopinath Balakrish ^{[1
]}

Takeda, Yoshifumi ^{[3
]}

Koley, Hemanta ^{[1
]}

机构：

[1] Natl Inst Cholera & Enter Dis, Kolkata 700010, India

[2] Natl Ctr Global Hlth & Med, Res Inst, Tokyo, Japan

[3] Okayama Univ Infect Dis India, Collaborat Res Ctr, Kolkata, India

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2011年 / 55卷 / 10期

关键词：

guinea pig colitis model; live non-invasive Shigella strain; oral immunization; SONNEI VACCINE STRAIN; FLEXNERI; 2A; SALMONELLA-TYPHIMURIUM; ANIMAL-MODEL; ORAL IMMUNIZATION; TYPE-1; VACCINE; IMMUNOGENICITY; SAFETY; EFFICACY; HUMANS;

D O I：

10.1111/j.1348-0421.2011.00371.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A non-invasive live transconjugant Shigella hybrid (LTSH Delta stx) strain was constructed from a Shiga toxin gene deleted mutant of Shigella dysenteriae 1 by introducing a plasmid vector pPR1347 that carried a lipopolysaccharide biosynthesis gene (rfb and rfc) of Salmonella typhimurium. In guinea pigs, four successive oral administrations of LTSH Delta stx showed complete protection against rectal challenge with wild type S. dysenteriae 1 strain. Exponential increase of the serum IgG and IgA titer against lipopolysaccharide of LTSH Delta stx was observed during immunization, peaked on day 28 and remained at that level until day 35 after the initiation of the immunization. In intestinal lavage of the immunized animals, significant increase of IgA titer against lipopolysaccharide of LTSH Delta stx was also observed. These data suggested that LTSH Delta stx could be a useful candidate to induce protective immunity against S. dysenteriae 1 infection.

引用

页码：683 / 693

页数：11

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