In vitro antimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite

被引：35

作者：

Grenho, L. ^{[1
,2
,3
]}

Barros, J. ^{[1
,2
,3
,4
]}

Ferreira, C. ^{[4
]}

Santos, V. R. ^{[5
]}

Monteiro, F. J. ^{[1
,2
,3
]}

Ferraz, M. P. ^{[1
,2
,6
]}

Cortes, M. E. ^{[5
]}

机构：

[1] Univ Porto, Inst Biomed Engn, INEB, P-4100 Oporto, Portugal

[2] Univ Porto, Inst Invest & Inovacao Saude I3S, P-4100 Oporto, Portugal

[3] Univ Porto, DEMM, Fac Engn, P-4100 Oporto, Portugal

[4] Univ Porto, LEPABE Lab Proc Engn Environm Biotechnol & Energy, Fac Engn, DEQ, P-4100 Oporto, Portugal

[5] Univ Fed Minas Gerais, Fac Odontol, Belo Horizonte, MG, Brazil

[6] Univ Fernando Pessoa, FP ENAS CEBIMED, Oporto, Portugal

来源：

BIOMEDICAL MATERIALS | 2015年 / 10卷 / 02期

关键词：

nanohydroxyapatite; propolis; antibacterial activity; cytocompatibility; BRAZILIAN RED PROPOLIS; CHEMICAL-COMPOSITION; BOTANICAL ORIGIN; ANTIBACTERIAL ACTIVITY; BIOLOGICAL-ACTIVITY; DIFFERENT REGIONS; CONSTITUENTS; ANTIBIOTICS; EXTRACTS; FILMS;

D O I：

10.1088/1748-6041/10/2/025004

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The high number of biomaterial associated infections demands new strategies to prevent this problem. In this study the suitability of nanohydroxyapatite (nanoHA)-based surfaces containing two Brazilian extracts of propolis (green and red ones) to prevent bacterial growth and biofilm formation, as well as its non-cytotoxic nature, was investigated. Optical density, colony forming units and MTT reduction assay were used to assess the materials' antibacterial activity against planktonic and sessile growth of Staphylococcus aureus. NanoHA matrix was able to absorb both types of propolis and the obtained results revealed the antibacterial effectiveness of the novel materials expressed as the reduction of bacterial growth and biofilm formation ability. Additionally, cell culture tests showed the growth of fibroblasts with high metabolic activity and without membrane damage. Therefore, these nanoHA-based surfaces containing natural products deriving from bees may be a promising bioactive biomaterial to be further studied with the aim of application to orthopaedic or dental devices.

引用

页数：8

共 51 条

[1] Effect of propolis on human fibroblasts from the pulp and periodontal ligament [J].