Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth

被引：52
作者：
Cappai, R [1 ]
Mok, SS
Galatis, D
Tucker, DF
Henry, A
Beyreuther, K
Small, DH
Masters, CL
机构：
[1] Univ Melbourne, Dept Pathol, Parkville, Vic 3052, Australia
[2] Mental Hlth Res Inst, Parkville, Vic 3052, Australia
[3] Univ Heidelberg, Ctr Mol Biol, D-69120 Heidelberg, Germany
来源：
FEBS LETTERS | 1999年 / 442卷 / 01期
关键词：
amyloid precursor-like protein 2; neuritogenesis;
D O I：
10.1016/S0014-5793(98)01635-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The human amyloid precursor-like protein 2 (APLP2) is a member of the Alzheimer's disease amyloid precursor protein (APP) gene family. The human APLP2 ectodomain (sAPLP2) was expressed in the yeast Pichia pastoris and the recombinant sAPLP2 was purified from the culture medium in a single step by metal-chelating Sepharose chromatography. The neuritotrophic activity of APLP2 was compared to the APP isoforms sAPP695 and sAPP751 on chick sympathetic neurones. APLP2 had neurite outgrowth-promoting activity similar to that of APP isoforms. This suggests that APP and APLP2 have a similar or related role and supports the idea of a redundancy in function between the APP-gene family proteins. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码：95 / 98
页数：4
相关论文
共 35 条
  • [1] Expression of a synthetic gene encoding the anticoagulant-antimetastatic protein ghilanten by the methylotropic yeast Pichia pastoris
    Brankamp, RG
    Sreekrishna, K
    Smith, PL
    Blankenship, DT
    Cardin, AD
    [J]. PROTEIN EXPRESSION AND PURIFICATION, 1995, 6 (06) : 813 - 820
  • [2] BUSH AI, 1994, J BIOL CHEM, V269, P26618
  • [3] BUSH AI, 1993, J BIOL CHEM, V268, P16109
  • [4] SECRETION OF NERVE GROWTH-FACTOR FROM SEPTUM STIMULATES NEURITE OUTGROWTH AND RELEASE OF THE AMYLOID PROTEIN-PRECURSOR OF ALZHEIMERS-DISEASE FROM HIPPOCAMPAL EXPLANTS
    CLARRIS, HJ
    NURCOMBE, V
    SMALL, DH
    BEYREUTHER, K
    MASTERS, CL
    [J]. JOURNAL OF NEUROSCIENCE RESEARCH, 1994, 38 (03) : 248 - 258
  • [5] RECENT ADVANCES IN THE EXPRESSION OF FOREIGN GENES IN PICHIA-PASTORIS
    CREGG, JM
    VEDVICK, TS
    RASCHKE, WC
    [J]. BIO-TECHNOLOGY, 1993, 11 (08): : 905 - 910
  • [6] ALZHEIMERS-DISEASE - INITIAL REPORT OF THE PURIFICATION AND CHARACTERIZATION OF A NOVEL CEREBROVASCULAR AMYLOID PROTEIN
    GLENNER, GG
    WONG, CW
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 120 (03) : 885 - 890
  • [7] Expression of human amyloid precursor protein ectodomains in Pichia pastoris: Analysis of culture conditions, purification, and characterization
    Henry, A
    Masters, CL
    Beyreuther, K
    Cappai, R
    [J]. PROTEIN EXPRESSION AND PURIFICATION, 1997, 10 (02) : 283 - 291
  • [8] THE BETA-A4 AMYLOID PRECURSOR PROTEIN-BINDING TO COPPER
    HESSE, L
    BEHER, D
    MASTERS, CL
    MULTHAUP, G
    [J]. FEBS LETTERS, 1994, 349 (01) : 109 - 116
  • [9] THE PRECURSOR OF ALZHEIMERS-DISEASE AMYLOID-A4 PROTEIN RESEMBLES A CELL-SURFACE RECEPTOR
    KANG, J
    LEMAIRE, HG
    UNTERBECK, A
    SALBAUM, JM
    MASTERS, CL
    GRZESCHIK, KH
    MULTHAUP, G
    BEYREUTHER, K
    MULLERHILL, B
    [J]. NATURE, 1987, 325 (6106) : 733 - 736
  • [10] SELECTIVE LOCALIZATION OF AMYLOID PRECURSOR-LIKE PROTEIN-1 IN THE CEREBRAL-CORTEX POSTSYNAPTIC DENSITY
    KIM, TW
    WU, K
    XU, JL
    MCAULIFFE, G
    TANZI, RE
    WASCO, W
    BLACK, IB
    [J]. MOLECULAR BRAIN RESEARCH, 1995, 32 (01): : 36 - 44
1234