In vivo reporter labeling of proteins via metabolic delivery of coenzyme A analogues

被引：82

作者：

Clarke, KM ^{[1
]}

Mercer, AC ^{[1
]}

La Clair, JJ ^{[1
]}

Burkart, MD ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 32期

关键词：

D O I：

10.1021/ja052911k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate site-specific reporter labeling of proteins within live cells. By accessing the coenzyme A (CoA) metabolic pathway with a cell-permeable pantetheine analogue, we deliver CoA-bound reporter molecules for post-translational protein modification in vivo. These methods may be applied to natural product pathway manipulation as well as applications in conventional molecular and cellular biology. Copyright © 2005 American Chemical Society.

引用

页码：11234 / 11235

页数：2

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