Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer

被引：333

作者：

Cheng, Ziyi ^{[1
]}

Choi, Namhyun ^{[1
]}

Wang, Rui ^{[1
]}

Lee, Sangyeop ^{[1
]}

Moon, Kyung Chul ^{[2
]}

Yoon, Soo-Young ^{[2
]}

Chen, Lingxin ^{[3
]}

Choo, Jaebum ^{[1
]}

机构：

[1] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea

[2] Korea Univ, Dept Lab Med, Coll Med, Seoul 152854, South Korea

[3] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China

来源：

ACS NANO | 2017年 / 11卷 / 05期

基金：

新加坡国家研究基金会; 中国国家自然科学基金;

关键词：

SERS-based immunoassay; multiplex detection; prostate cancer; free to total PSA ratio; simultaneous detection; TOTAL PSA; BIOMARKER DETECTION; SERS; NANOPARTICLES; MARKERS; PROTEIN; ASSAY; DIFFERENTIATION; NANOPROBES; PERCENTAGE;

D O I：

10.1021/acsnano.7b01536

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Accurate analysis of specific biomarkers in clinical serum is essential for early diagnosis and treatment of cancer. Here, a surface-enhanced Raman scattering (SERS)-based immunoassay, using magnetic beads and SERS nano tags, was developed for the determination of free to total (f/t) prostate specific antigen (PSA) ratio to improve the diagnostic performance of prostate cancer. To assess the clinical applicability of the proposed method, SERS-based assays for the simultaneous detection of dual PSA markers, free PSA (f-PSA) and complexed PSA (c-PSA), were performed for clinical samples in the gray zone between 4.0 and 10.0 ng/mL. Our assay results for f/t PSA ratio showed a good linear correlation with those measured using the electrochemiluminescence (ECL) system installed in the clinical laboratory of the University Hospital. In addition, the simultaneous assay provided better precision than parallel assays for the detection of f-PSA and c-PSA in 13 clinical serum samples. Therefore, our SERS-based assay for simultaneous detection of dual PSA markers in clinical fluids has strong potential for application in the accurate diagnosis of prostate cancer.

引用

页码：4926 / 4933

页数：8

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