Spherical silver nanoparticles in the detection of thermally denatured collagens

被引：6

作者：

Ahumada, Manuel ^{[1
,2
]}

McLaughlin, Sarah ^{[1
]}

Pacioni, Natalia L. ^{[3
,4
]}

Alarcon, Emilio I. ^{[1
,5
]}

机构：

[1] Univ Ottawa, Inst Heart, Div Cardiac Surg, Bionanomat Chem & Engn Lab, 40 Ruskin St,Rm H5229, Ottawa, ON K1Y 4W7, Canada

[2] Univ Santiago Chile, Lab Cinet & Fotoquim, Dept Ciencias Ambiente, Fac Quim & Biol, Ave Libertador Bernardo Ohiggins 3363, Santiago 8370071, Chile

[3] Consejo Nacl Invest Cient & Tecn, INFIQC, Fac Ciencias Quim, Dept Quim Organ, Haya Torre & Medina Allende S-N,X5000HUA, RA-5000 Cordoba, Argentina

[4] Univ Nacl Cordoba, Haya Torre & Medina Allende S-N,X5000HUA, RA-5000 Cordoba, Argentina

[5] Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, Ottawa, ON K1Y 4W7, Canada

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2016年 / 408卷 / 08期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Collagen; Nanoparticles/Nanotechnology; Surface plasmon band; PROTEIN; GOLD;

D O I：

10.1007/s00216-016-9330-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a rapid colorimetric method to determine the concentration of denatured collagen in solution, which is based on the collagen-silver nanoparticle corona formation. Using the proposed method, the lowest detectable concentration of denatured collagen protein in a solution of pure collagen was 14.7, 8.5, and 8.6 mu g mL(-1) for porcine (PCOL), rat tail (RCOL), and type I human recombinant (HCOL) collagen, respectively.

引用

页码：1993 / 1996

页数：4

共 23 条

[11] Hollow core photonic crystal fiber for monitoring leukemia cells using surface enhanced Raman scattering (SERS) [J].