Spherical silver nanoparticles in the detection of thermally denatured collagens

被引：6
作者：
Ahumada, Manuel [1 ,2 ]
McLaughlin, Sarah [1 ]
Pacioni, Natalia L. [3 ,4 ]
Alarcon, Emilio I. [1 ,5 ]
机构：
[1] Univ Ottawa, Inst Heart, Div Cardiac Surg, Bionanomat Chem & Engn Lab, 40 Ruskin St,Rm H5229, Ottawa, ON K1Y 4W7, Canada
[2] Univ Santiago Chile, Lab Cinet & Fotoquim, Dept Ciencias Ambiente, Fac Quim & Biol, Ave Libertador Bernardo Ohiggins 3363, Santiago 8370071, Chile
[3] Consejo Nacl Invest Cient & Tecn, INFIQC, Fac Ciencias Quim, Dept Quim Organ, Haya Torre & Medina Allende S-N,X5000HUA, RA-5000 Cordoba, Argentina
[4] Univ Nacl Cordoba, Haya Torre & Medina Allende S-N,X5000HUA, RA-5000 Cordoba, Argentina
[5] Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, Ottawa, ON K1Y 4W7, Canada
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2016年 / 408卷 / 08期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Collagen; Nanoparticles/Nanotechnology; Surface plasmon band; PROTEIN; GOLD;
D O I：
10.1007/s00216-016-9330-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a rapid colorimetric method to determine the concentration of denatured collagen in solution, which is based on the collagen-silver nanoparticle corona formation. Using the proposed method, the lowest detectable concentration of denatured collagen protein in a solution of pure collagen was 14.7, 8.5, and 8.6 mu g mL(-1) for porcine (PCOL), rat tail (RCOL), and type I human recombinant (HCOL) collagen, respectively.
引用
收藏
页码：1993 / 1996
页数：4
相关论文
共 23 条
[1]   Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds [J].
Alarcon, Emilio I. ;
Udekwu, Klas I. ;
Noel, Christopher W. ;
Gagnon, Luke B. -P. ;
Taylor, Patrick K. ;
Vulesevic, Branka ;
Simpson, Madeline J. ;
Gkotzis, Spyridon ;
Islam, M. Mirazul ;
Lee, Chyan-Jang ;
Richter-Dahlfors, Agneta ;
Mah, Thien-Fah ;
Suuronen, Erik J. ;
Scaiano, Juan C. ;
Griffith, May .
NANOSCALE, 2015, 7 (44) :18789-18798
[2]   Human serum albumin as protecting agent of silver nanoparticles: role of the protein conformation and amine groups in the nanoparticle stabilization [J].
Alarcon, Emilio I. ;
Bueno-Alejo, Carlos J. ;
Noel, Christopher W. ;
Stamplecoskie, Kevin G. ;
Pacioni, Natalia L. ;
Poblete, Horacio ;
Scaiano, J. C. .
JOURNAL OF NANOPARTICLE RESEARCH, 2013, 15 (01)
[3]   The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles [J].
Alarcon, Emilio I. ;
Udekwu, Klas ;
Skog, Marten ;
Pacioni, Natalia L. ;
Stamplecoskie, Kevin G. ;
Gonzalez-Bejar, Maria ;
Polisetti, Naresh ;
Wickham, Abeni ;
Richter-Dahlfors, Agneta ;
Griffith, May ;
Scaiano, Juan C. .
BIOMATERIALS, 2012, 33 (19) :4947-4956
[4]  
Barringer SA, 2006, ADVANCES IN MICROWAVE AND RADIO FREQUENCY PROCESSING, P107
[5]   Collagen-based silver nanoparticles for biological applications: synthesis and characterization [J].
Cardoso, Vinicius S. ;
Quelemes, Patrick V. ;
Amorin, Adriany ;
Primo, Fernando Lucas ;
Gobo, Graciely Gomides ;
Tedesco, Antonio C. ;
Mafud, Ana C. ;
Mascarenhas, Yvonne P. ;
Correa, Jose Raimundo ;
Kuckelhaus, Selma A. S. ;
Eiras, Carla ;
Leite, Jose Roberto S. A. ;
Silva, Durcilene ;
dos Santos Junior, Jose Ribeiro .
JOURNAL OF NANOBIOTECHNOLOGY, 2014, 12 :1-9
[6]  
Chen HH, 2015, EXP TECHNIQUES, DOI [10.1111/ext.12138, DOI 10.1111/EXT.12138]
[7]  
Cho EC, 2011, NAT NANOTECHNOL, V6, P385, DOI [10.1038/nnano.2011.58, 10.1038/NNANO.2011.58]
[8]   Lysozyme Catalyzes the Formation of Antimicrobial Silver Nanoparticles [J].
Eby, D. Matthew ;
Schaeublin, Nicole M. ;
Farrington, Karen E. ;
Hussain, Saber M. ;
Johnson, Glenn R. .
ACS NANO, 2009, 3 (04) :984-994
[9]   Gold nanoparticles in delivery applications [J].
Ghosh, Partha ;
Han, Gang ;
De, Mrinmoy ;
Kim, Chae Kyu ;
Rotello, Vincent M. .
ADVANCED DRUG DELIVERY REVIEWS, 2008, 60 (11) :1307-1315
[10]   A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel-based repair cartilage [J].
Hoemann, CD ;
Sun, J ;
Chrzanowski, V ;
Buschmann, MD .
ANALYTICAL BIOCHEMISTRY, 2002, 300 (01) :1-10
123